Home LiteratureArticle Details

PMID: 7529928 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cyclic AMP stimulates K+ channel activity in mesophyll cells of Vicia faba L.

Plant physiology ·Vol. 106 ·No. 3 ·1994-11-00 ·Pages 957-61

Li W, Luan S, Schreiber SL, Assmann SM

Abstract

Whole-cell patch-clamp recordings from Vicia faba mesophyll protoplasts reveal that outward K+ current is increased in a dose-dependent fashion by intracellular application of cAMP. The enhancement of the outward current by cAMP is specific and it cannot be mimicked by a series of nucleotides that includes AMP, cGMP, and GMP. The enhancement is evoked by micromolar concentrations of cAMP in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methyl-xanthine. PKI or Walsh inhibitor, a specific peptide inhibitor of cAMP-dependent protein kinase (PKA), inhibits the outward K+ current. Adenosine 3',5'-phosphothioate, a competitive inhibitor of PKA, has a similar effect. Conversely, the catalytic subunit of PKA (cAMP independent) from bovine brain enhances the magnitude of the outward K+ current in the absence of added cAMP. Our results indicate that cAMP modulates K+ channel activity in mesophyll cells and suggest that this modulation occurs through a cAMP-regulated protein kinase.

MeSH Terms

1-Methyl-3-isobutylxanthine/pharmacology 8-Bromo Cyclic Adenosine Monophosphate/pharmacology Adenosine/analogs & derivatives,pharmacology Animals Brain/enzymology Bucladesine/pharmacology Cattle Cyclic AMP/pharmacology Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors,pharmacology Cyclic GMP/pharmacology Electric Stimulation Fabaceae/physiology Guanosine Monophosphate/pharmacology Intercellular Signaling Peptides and Proteins Kinetics Macromolecular Substances Membrane Potentials/drug effects Organothiophosphorus Compounds/pharmacology Peptides/pharmacology Plants, Medicinal Potassium Channels/drug effects,physiology

Chemicals

Intercellular Signaling Peptides and Proteins Macromolecular Substances Organothiophosphorus Compounds Peptides Potassium Channels Walsh peptide adenosine 3',5'-phosphothioate 8-Bromo Cyclic Adenosine Monophosphate Bucladesine Guanosine Monophosphate Cyclic AMP Cyclic AMP-Dependent Protein Kinases Cyclic GMP Adenosine 1-Methyl-3-isobutylxanthine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Li W

Biological Laboratory, Harvard University, Cambridge, Massachusetts 02138.

Luan S

Schreiber S L

Assmann S M

References (15)

15 references, click to expand

Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
Science. 1992 Dec 4;258(5088):1654-8 PMID: 8966547
Cyclic nucleotide-dependent protein kinases. IV. Widespread occurrence of adenosine 3',5'-monophosphate-dependent protein kinase in various tissues and phyla of the animal kingdom.
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1349-55 PMID: 4393915
Regulation of Ca2+-dependent K+-channel activity in tracheal myocytes by phosphorylation.
Nature. 1989 Sep 14;341(6238):152-4 PMID: 2550823
Interaction of cAMP derivatives with the 'stable' cAMP-binding site in the cAMP-dependent protein kinase type I.
Eur J Biochem. 1982 Feb;122(1):95-9 PMID: 6277633
cDNA Sequence of PsPK5, a Protein Kinase Homolog from Pisum sativum L.
Plant Physiol. 1992 Oct;100(2):1072-4 PMID: 16653022
Cyclic AMP and other signals controlling cell development and differentiation in Dictyostelium.
Annu Rev Biochem. 1987;56:853-79 PMID: 3039908
Evidence for protein phosphatase 1 and 2A regulation of K+ channels in two types of leaf cells.
Plant Physiol. 1994 Nov;106(3):963-70 PMID: 7824661
Sympathetic regulation of cardiac calcium current is due exclusively to cAMP-dependent phosphorylation.
Nature. 1991 Jun 13;351(6327):573-6 PMID: 1710784
Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa).
Biochem J. 1988 Feb 1;249(3):807-11 PMID: 3128270
Protein kinase injection reduces voltage-dependent potassium currents.
Science. 1983 Jan 21;219(4582):303-6 PMID: 6294830
Characterization of a G-protein-regulated outward K+ current in mesophyll cells of vicia faba L.
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):262-6 PMID: 8419932
Cloning and expression in yeast of a plant potassium ion transport system.
Science. 1992 May 1;256(5057):663-5 PMID: 1585180
Peptide inhibitors of cAMP-dependent protein kinase.
Methods Enzymol. 1988;159:173-83 PMID: 2842583
A cyclic AMP-activated K+ channel in Drosophila larval muscle is persistently activated in dunce.
Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):557-60 PMID: 1846445
Molecular cloning of plant transcripts encoding protein kinase homologs.
Proc Natl Acad Sci U S A. 1989 May;86(9):3140-4 PMID: 2541432

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.106.3.957

Published

1994-11-00

Pages

957-61

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC159618

Subset

Grants

NIGMS NIH HHS · GM-38627 · United States

External Links

PubMed source DOI: 10.1104/pp.106.3.957 PMC Full Text View journal details

Analysis Services

Cyclic AMP stimulates K+ channel activity in mesophyll cells of Vicia faba L.

Contact

WeChat Official Account