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PMID: 7524079 Published · ppublish English Comparative Study Journal Article

Temporal differences in the activation of three classes of non-transmembrane protein tyrosine kinases following B-cell antigen receptor surface engagement.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 20 ·1994-09-27 ·Pages 9524-8

Saouaf SJ, Mahajan S, Rowley RB, Kut SA, Fargnoli J, Burkhardt AL, Tsukada S, Witte ON, Bolen JB

Abstract

We evaluated in WEHI 231 B cells the time-dependent responses of Lyn, Blk, Btk, Syk, and three members of the Jak family of protein tyrosine kinases following antibody-mediated surface engagement of the B-cell antigen receptor. Our results show that the enzyme activities of Lyn and Blk were stimulated within seconds of antigen receptor engagement and correlated with the initial tyrosine phosphorylation of the Ig alpha and Ig beta subunits of the B-cell antigen receptor. Btk enzyme activity was also transiently stimulated and was maximal at approximately 5 min after B-cell receptor surface binding. Syk activity gradually increased to a maximum at 10-30 min following receptor ligation and was found to parallel the association of Syk with the tyrosine phosphorylated Ig alpha and Ig beta subunits of the receptor. While the specific activities of the Jak1, Jak2, and Tyk2 protein tyrosine kinases were unaltered following B-cell receptor ligation, the abundance of Jak1 and Jak2 were increased 3- to 4-fold within 10 min of receptor engagement. These results demonstrate that multiple families of non-transmembrane protein tyrosine kinases are temporally regulated during the process of B-cell antigen receptor-initiated intracellular signal transduction.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/enzymology,immunology Cell Line Conserved Sequence Enzyme Activation Immunoglobulin G/pharmacology Kinetics Mice Molecular Sequence Data Phosphoproteins/isolation & purification,metabolism Phosphotyrosine Protein-Tyrosine Kinases/metabolism Receptors, Antigen, B-Cell/physiology Time Factors Tyrosine/analogs & derivatives,analysis,metabolism
Chemicals
Immunoglobulin G Phosphoproteins Receptors, Antigen, B-Cell Phosphotyrosine Tyrosine Protein-Tyrosine Kinases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Saouaf S J
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
Mahajan S
Rowley R B
Kut S A
Fargnoli J
Burkhardt A L
Tsukada S
Witte O N
Bolen J B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.20.9524
Published
1994-09-27
Pages
9524-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44845
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.20.9524 PMC Full Text View journal details
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