Home LiteratureArticle Details
PMID: 7513081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nuclear factor that binds purine-rich, single-stranded oligonucleotides derived from S1-sensitive elements upstream of the CFTR gene and the MUC1 gene.

Nucleic acids research ·Vol. 22 ·No. 7 ·1994-04-11 ·Pages 1138-46

Hollingsworth MA, Closken C, Harris A, McDonald CD, Pahwa GS, Maher LJ

Abstract

We have identified two regions of non-random purine/pyrimidine strand asymmetry that were nearly identical in sequence in the 5' flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purine-rich strand of this region, but not to the pyrimidine-rich strands or to double-stranded oligonucleotides with corresponding purine/pyrimidine strand asymmetry.

Related Genes
CFTR MUC1
MeSH Terms
Base Sequence Binding, Competitive Cystic Fibrosis/genetics Cystic Fibrosis Transmembrane Conductance Regulator DNA, Single-Stranded/chemistry,metabolism Humans Hydrogen-Ion Concentration Membrane Glycoproteins/genetics Molecular Sequence Data Mucin-1 Mucins/genetics Nuclear Proteins/metabolism Nucleic Acid Conformation Oligodeoxyribonucleotides/chemistry,metabolism Purines/metabolism Repetitive Sequences, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases/metabolism Trans-Activators/metabolism Tumor Cells, Cultured Ultraviolet Rays
Chemicals
CFTR protein, human DNA, Single-Stranded Membrane Glycoproteins Mucin-1 Mucins Nuclear Proteins Oligodeoxyribonucleotides Purines Trans-Activators Cystic Fibrosis Transmembrane Conductance Regulator Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hollingsworth M A
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
Closken C
Harris A
McDonald C D
Pahwa G S
Maher L J
References (23)
23 references, click to expand
  1. A pH-dependent structural transition in the homopurine-homopyrimidine tract in superhelical DNA.
    J Biomol Struct Dyn. 1985 Oct;3(2):327-38 PMID: 3917024
  2. Ribonucleoprotein and protein factors bind to an H-DNA-forming c-myc DNA element: possible regulators of the c-myc gene.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9682-6 PMID: 2690070
  3. A single-stranded DNA binding protein from S. cerevisiae specifically recognizes the T-rich strand of the core sequence of ARS elements and discriminates against mutant sequences.
    EMBO J. 1991 Apr;10(4):981-5 PMID: 2009863
  4. Identification of novel single-stranded d(TC)n binding proteins in several mammalian species.
    Nucleic Acids Res. 1991 Feb 25;19(4):949-53 PMID: 2017376
  5. Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein.
    Nucleic Acids Res. 1991 Oct 11;19(19):5237-45 PMID: 1681508
  6. Characterization of the promoter region of the cystic fibrosis transmembrane conductance regulator gene.
    J Biol Chem. 1991 Dec 25;266(36):24471-6 PMID: 1722205
  7. A CT promoter element binding protein: definition of a double-strand and a novel single-strand DNA binding motif.
    Nucleic Acids Res. 1992 Jan 11;20(1):111-6 PMID: 1738588
  8. The HeLa Pur factor binds single-stranded DNA at a specific element conserved in gene flanking regions and origins of DNA replication.
    Mol Cell Biol. 1992 Mar;12(3):1257-65 PMID: 1545807
  9. Characterization of cis-acting elements regulating transcription of the human DF3 breast carcinoma-associated antigen (MUC1) gene.
    Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):282-6 PMID: 8419933
  10. Analysis of the tissue-specific promoter of the MUC1 gene.
    J Biol Chem. 1993 May 5;268(13):9917-26 PMID: 8387509
  11. Identification of a single-stranded DNA-binding protein that interacts with an S1 nuclease-sensitive region in the platelet-derived growth factor A-chain gene promoter.
    J Biol Chem. 1993 May 15;268(14):10681-5 PMID: 8486717
  12. Expression of the cystic fibrosis gene in human foetal tissues.
    Hum Mol Genet. 1993 Mar;2(3):213-8 PMID: 7684639
  13. Characterization of the cystic fibrosis transmembrane conductance regulator promoter region. Chromatin context and tissue-specificity.
    J Biol Chem. 1993 Jul 25;268(21):15912-21 PMID: 7688000
  14. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells.
    J Cell Sci. 1993 Jun;105 ( Pt 2):417-22 PMID: 7691840
  15. The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72 PMID: 6256738
  16. Cruciform structures in supercoiled DNA.
    Nature. 1981 Feb 5;289(5797):466-70 PMID: 7464915
  17. An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
    Cell. 1982 Jun;29(2):609-22 PMID: 6288265
  18. Control of ColE1 plasmid replication: the process of binding of RNA I to the primer transcript.
    Cell. 1984 Oct;38(3):861-70 PMID: 6207934
  19. An unusually long poly(purine)-poly(pyrimidine) sequence is located upstream from the human thyroglobulin gene.
    Nucleic Acids Res. 1985 Jul 25;13(14):5127-44 PMID: 2991855
  20. Influence of DNA sequence on the formation of non-B right-handed helices in oligopurine.oligopyrimidine inserts in plasmids.
    J Biol Chem. 1988 May 25;263(15):7386-96 PMID: 2835375
  21. Single strands, triple strands, and kinks in H-DNA.
    Science. 1988 Sep 30;241(4874):1791-6 PMID: 3175620
  22. The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences.
    FASEB J. 1988 Nov;2(14):2939-49 PMID: 3053307
  23. Effect of length, supercoiling, and pH on intramolecular triplex formation. Multiple conformers at pur.pyr mirror repeats.
    J Biol Chem. 1990 Jun 25;265(18):10652-8 PMID: 2355014
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.7.1138
Published
1994-04-11
Pages
1138-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523634
Subset
IM
Grants
NCI NIH HHS · CA57362 · United States
NIDDK NIH HHS · DK44762 · United States
NIGMS NIH HHS · GM47814 · United States
External Links
PubMed source DOI: 10.1093/nar/22.7.1138 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2