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PMID: 7508460 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments.

Journal of clinical microbiology ·Vol. 31 ·No. 12 ·1993-12-00 ·Pages 3340-3

Eyers M, Chapelle S, Van Camp G, Goossens H, De Wachter R

Abstract

By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. One primer pair selectively detected all thermophilic Campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species Campylobacter coli, Campylobacter jejuni subsp. jejuni, Campylobacter lari, and Campylobacter upsaliensis. All primer sets were tested successfully on a large number of clinical isolates.

MeSH Terms
Base Sequence Campylobacter/classification,genetics,isolation & purification Campylobacter coli/genetics Campylobacter jejuni/genetics DNA Primers/genetics DNA, Bacterial/genetics Evaluation Studies as Topic Humans Molecular Sequence Data Polymerase Chain Reaction/methods,statistics & numerical data RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics Sensitivity and Specificity Species Specificity
Chemicals
DNA Primers DNA, Bacterial RNA, Bacterial RNA, Ribosomal, 23S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Eyers M
Department Biochemie, Universiteit Antewerpen, UIA, Belgium.
Chapelle S
Van Camp G
Goossens H
De Wachter R
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.12.3340-3343.1993
Published
1993-12-00
Pages
3340-3
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC266428
Subset
IM
Corrections
ErratumIn
PMID: 7915724
External Links
PubMed source DOI: 10.1128/jcm.31.12.3340-3343.1993 PMC Full Text View journal details
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