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PMID: 7500037 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

TAP-independent, beta 2-microglobulin-dependent surface expression of functional mouse CD1.1.

The Journal of experimental medicine ·Vol. 182 ·No. 6 ·1995-12-01 ·Pages 1913-9

Brutkiewicz RR, Bennink JR, Yewdell JW, Bendelac A

Abstract

CD1 molecules consist of beta 2-microglobulin (beta 2m) noncovalently complexed to a non-major histocompatibility complex (MHC)-encoded monomorphic integral membrane protein homologous to MHC class I alpha chains. Little is known about the requirements for cell surface expression and T cell recognition of CD1. We inserted the mouse CD1.1 gene into vaccinia virus to create a recombinant virus expressing CD1.1 under the control of a viral promoter. Using this recombinant virus to infect normal or mutant cell lines, we found that the expression of molecules reactive with the CD1.1-specific monoclonal antibody 3C11 requires the expression of beta 2m but was not affected by the absence of the MHC-encoded peptide transporter (TAP). Consistent with these results, IL-2 production by the mCD1.1-specific T cell hybridoma DN32.D3 was induced by thymocytes from normal mice or mice with a homozygous deletion of the TAP1 gene, but not by thymocytes from mice with a homozygous deletion of the beta 2m gene. These results indicate that expression of functional mCD1.1 occurs in a beta 2m-dependent, TAP-independent manner.

MeSH Terms
ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP Binding Cassette Transporter, Subfamily B, Member 3 ATP-Binding Cassette Transporters/metabolism Animals Antigens, CD1/metabolism Biological Transport Cells, Cultured Humans Lymphocyte Activation Membrane Glycoproteins/metabolism Mice Mice, Inbred C57BL Recombinant Proteins/metabolism T-Lymphocytes/immunology Vaccinia virus beta 2-Microglobulin/metabolism
Chemicals
ATP Binding Cassette Transporter, Subfamily B, Member 2 ATP Binding Cassette Transporter, Subfamily B, Member 3 ATP-Binding Cassette Transporters Antigens, CD1 Membrane Glycoproteins Recombinant Proteins TAP1 protein, human Tap1 protein, mouse Tap2 protein, mouse beta 2-Microglobulin TAP2 protein, human
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brutkiewicz R R
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0440, USA.
Bennink J R
Yewdell J W
Bendelac A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.6.1913
Published
1995-12-01
Pages
1913-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192266
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.182.6.1913 PMC Full Text View journal details
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