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PMID: 8496682 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Class I molecules retained in the endoplasmic reticulum bind antigenic peptides.

The Journal of experimental medicine ·Vol. 177 ·No. 6 ·1993-06-01 ·Pages 1633-41

Lapham CK, Bacík I, Yewdell JW, Kane KP, Bennink JR

Abstract

We isolated major histocompatibility complex (MHC)-specific viral peptides from cells infected with influenza virus in the continuous presence of the drug brefeldin A, which blocks exocytosis of newly synthesized MHC class I molecules. MHC-specific peptides were also isolated from cells expressing mouse Kd class I MHC molecules whose cytoplasmic domain was substituted by that of the adenovirus E3/19K glycoprotein. This molecule was retained in an intracellular pre-Golgi complex compartment as demonstrated by immunocytochemical and biochemical means. Since we show that intracellular association of antigenic peptides with such retained class I molecules is necessary for their isolation from cellular extracts, this provides direct evidence that naturally processed peptides associate with class I MHC molecules in an early intracellular exocytic compartment.

MeSH Terms
Animals Antigens, Viral/metabolism Brefeldin A Cell Line Cyclopentanes/pharmacology Endoplasmic Reticulum/metabolism Exocytosis Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/metabolism Histocompatibility Antigens Class I/metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Nucleocapsid Proteins Nucleoproteins/metabolism Peptides/immunology,metabolism RNA-Binding Proteins Viral Core Proteins/metabolism
Chemicals
Antigens, Viral Cyclopentanes Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Histocompatibility Antigens Class I NP protein, Influenza A virus Nucleocapsid Proteins Nucleoproteins Peptides RNA-Binding Proteins Viral Core Proteins Brefeldin A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lapham C K
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Bacík I
Yewdell J W
Kane K P
Bennink J R
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.177.6.1633
Published
1993-06-01
Pages
1633-41
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191061
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.177.6.1633 PMC Full Text View journal details
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