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PMID: 7487023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains.

Applied and environmental microbiology ·Vol. 61 ·No. 8 ·1995-08-00 ·Pages 2898-904

Rivera IG, Chowdhury MA, Huq A, Jacobs D, Martins MT, Colwell RR

Abstract

Enterobacterial repetitive intergenic consensus (ERIC) sequence polymorphism was studied in Vibrio Cholerae strains isolated before and after the cholera epidemic in Brazil (in 1991), along with epidemic strains from Peru, Mexico, and India, by PCR. A total of 17 fingerprint patterns (FPs) were detected in the V. cholerae strains examined; 96.7% of the toxigenic V. cholerae O1 strains and 100% of the O139 serogroup strains were found to belong to the same FP group comprising four fragments (FP1). The nontoxigenic V. cholerae O1 also yielded four fragments but constituted a different FP group (FP2). A total of 15 different patterns were observed among the V. cholerae non-O1 strains. Two patterns were observed most frequently for V. cholerae non-01 strains, 25% of which have FP3, with five fragments, and 16.7% of which have FP4, with two fragments. Three fragments, 1.75, 0.79, and 0.5 kb, were found to be common to both toxigenic and nontoxigenic V. cholerae O1 strains as well as to group FP3, containing V. cholerae non-O1 strains. Two fragments of group FP3, 1.3 and 1.0 kb, were present in FP1 and FP2 respectively. The 0.5-kb fragment was common to all strains and serogroups of V. cholerae analyzed. It is concluded from the results of this study, based on DNA FPs of environmental isolates, that it is possible to detect an emerging virulent strain in a cholera-endemic region. ERIC-PCR constitutes a powerful tool for determination of the virulence potential of V. cholerae O1 strains isolated in surveillance programs and for molecular epidemiological investigations.

MeSH Terms
Bacterial Toxins/biosynthesis Base Sequence Cholera/epidemiology,microbiology Consensus Sequence DNA Fingerprinting DNA Primers/genetics DNA, Bacterial/genetics Disease Outbreaks Genome, Bacterial Humans Molecular Epidemiology Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Repetitive Sequences, Nucleic Acid Serotyping Vibrio cholerae/classification,genetics,pathogenicity Virulence/genetics
Chemicals
Bacterial Toxins DNA Primers DNA, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rivera I G
Department of Microbiology, University of Maryland, College Park, USA.
Chowdhury M A
Huq A
Jacobs D
Martins M T
Colwell R R
References (38)
38 references, click to expand
  1. Non-cholera vibrio infections in the United States. Clinical, epidemiologic, and laboratory features.
    Ann Intern Med. 1978 May;88(5):602-6 PMID: 646241
  2. Bengal: El Tor cholera vibrio in a new robe.
    Mem Inst Oswaldo Cruz. 1994 Jan-Mar;89(1):115-6 PMID: 7823808
  3. Rapid isolation of high molecular weight plant DNA.
    Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PMID: 7433111
  4. Epidemiological usefulness of changes in hemolytic activity of Vibrio cholerae biotype El Tor during the seventh pandemic.
    J Clin Microbiol. 1981 Jan;13(1):126-9 PMID: 7462409
  5. Vibrio cholerae meningitis in a neonate.
    J Pediatr. 1981 Jun;98(6):940-2 PMID: 7229800
  6. Molecular characterization of environmental and nontoxigenic strains of Vibrio cholerae.
    Infect Immun. 1981 May;32(2):661-7 PMID: 7251142
  7. Molecular epidemiological studies of United States Gulf Coast Vibrio cholerae strains: integration site of mutator vibriophage VcA-3.
    Infect Immun. 1983 Oct;42(1):224-30 PMID: 6618665
  8. Isolation of nontoxigenic Vibrio cholerae O group 1 from a patient with severe gastrointestinal disease.
    J Clin Microbiol. 1984 Feb;19(2):296-7 PMID: 6199370
  9. Persistence of plasmids, cholera toxin genes, and prophage DNA in classical Vibrio cholerae O1.
    Infect Immun. 1984 Jul;45(1):222-6 PMID: 6329956
  10. Molecular cloning of the hemolysin determinant from Vibrio cholerae El Tor.
    J Bacteriol. 1984 Oct;160(1):239-44 PMID: 6090415
  11. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.
    Appl Environ Microbiol. 1986 May;51(5):873-84 PMID: 2425735
  12. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  13. Molecular epidemiology of Vibrio cholerae in Hong Kong.
    J Clin Microbiol. 1989 Aug;27(8):1900-2 PMID: 2570082
  14. Experimental non-O group 1 Vibrio cholerae gastroenteritis in humans.
    J Clin Invest. 1990 Mar;85(3):697-705 PMID: 2312721
  15. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
    Nucleic Acids Res. 1990 Nov 25;18(22):6531-5 PMID: 1979162
  16. Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns.
    Res Microbiol. 1990 Jul-Aug;141(6):645-57 PMID: 2284500
  17. Non-O group 1 Vibrio cholerae: a look at the epidemiology of an occasional pathogen.
    Epidemiol Rev. 1990;12:179-91 PMID: 2286218
  18. Restriction fragment length polymorphism analysis of Vibrio cholerae strains associated with a cholera outbreak in Hong Kong.
    J Clin Microbiol. 1991 May;29(5):1058-9 PMID: 1676035
  19. ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteria.
    Mol Microbiol. 1991 Apr;5(4):825-34 PMID: 1713281
  20. Genetic diversity among toxigenic and nontoxigenic Vibrio cholerae O1 isolated from the Western Hemisphere.
    Epidemiol Infect. 1991 Aug;107(1):225-33 PMID: 1879486
  21. Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers.
    Nucleic Acids Res. 1991 Oct 11;19(19):5275-9 PMID: 1923811
  22. Identification of Vibrio cholerae by enzyme electrophoresis.
    Trans R Soc Trop Med Hyg. 1991 Jul-Aug;85(4):544-7 PMID: 1755069
  23. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.
    Nucleic Acids Res. 1991 Dec 25;19(24):6823-31 PMID: 1762913
  24. Short, interspersed repetitive DNA sequences in prokaryotic genomes.
    J Bacteriol. 1992 Jul;174(14):4525-9 PMID: 1624445
  25. Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria.
    Appl Environ Microbiol. 1992 Jul;58(7):2180-7 PMID: 1637156
  26. Development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based co-agglutination test for direct detection of Vibrio cholerae 01.
    FEMS Microbiol Lett. 1992 Oct 15;76(3):215-9 PMID: 1427010
  27. Ecology and distribution of toxigenic Vibrio cholerae in aquatic environments of a temperate region.
    Microbios. 1992;72(292-293):203-13 PMID: 1488021
  28. Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake.
    Int J Syst Bacteriol. 1993 Jan;43(1):77-83 PMID: 7678983
  29. Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.
    Appl Environ Microbiol. 1993 Feb;59(2):556-60 PMID: 8434922
  30. The molecular epidemiology of cholera in Latin America.
    J Infect Dis. 1993 Mar;167(3):621-6 PMID: 7680060
  31. Comparison of Vibrio cholerae O139 with V. cholerae O1 classical and El Tor biotypes.
    Infect Immun. 1994 Apr;62(4):1504-6 PMID: 7510671
  32. Genome size and restriction fragment length polymorphism analysis of Vibrio cholerae strains belonging to different serovars and biotypes.
    FEMS Microbiol Lett. 1994 Jan 15;115(2-3):329-34 PMID: 7908004
  33. Vibrio cholerae O139 synonym bengal is closely related to Vibrio cholerae El Tor but has important differences.
    Infect Immun. 1994 May;62(5):2108-10 PMID: 8168977
  34. A gene for the enterotoxin zonula occludens toxin is present in Vibrio mimicus and Vibrio cholerae O139.
    FEMS Microbiol Lett. 1994 Jun 15;119(3):377-80 PMID: 8050719
  35. Cholera DFA: an improved direct fluorescent monoclonal antibody staining kit for rapid detection and enumeration of Vibrio cholerae O1.
    FEMS Microbiol Lett. 1994 Jul 1;120(1-2):143-8 PMID: 8056287
  36. Molecular characterization of Vibrio cholerae O1 strains by pulsed-field gel electrophoresis.
    J Clin Microbiol. 1994 Jul;32(7):1685-90 PMID: 7929758
  37. Potentially pathogenic vibrios associated with mussels from a tropical region on the Atlantic coast of Brazil.
    J Appl Bacteriol. 1994 Sep;77(3):281-7 PMID: 7989253
  38. Fulminating systemic infection caused by Vibrio cholerae species which does not agglutinate with 0-1 V. cholerae antiserum.
    Med J Aust. 1980 Jun 28;1(13):659-61 PMID: 7421625
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.61.8.2898-2904.1995
Published
1995-08-00
Pages
2898-904
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC167567
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.61.8.2898-2904.1995 PMC Full Text View journal details
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