Molecular epidemiology of Vibrio cholerae in Hong Kong.

Journal of clinical microbiology ·Vol. 27 ·No. 8 ·1989-08-00 ·Pages 1900-2

Yam WC, Lung ML, Ng KY, Ng MH

Abstract

We studied restriction fragment length polymorphism of the enterotoxin genes of isolates of Vibrio cholerae El Tor, indistinguishable by bacteriophage typing, which were collected in Hong Kong since 1978. Using this approach, we could distinguish indigenous and exogenous strains obtained from different sources and epidemiological settings.

MeSH Terms

Autoradiography Bacteriophage Typing Blotting, Southern Cholera/epidemiology,microbiology DNA Probes DNA, Bacterial/genetics Electrophoresis, Agar Gel Hong Kong Humans Nucleic Acid Hybridization Polymorphism, Genetic Polymorphism, Restriction Fragment Length Restriction Mapping Sequence Homology, Nucleic Acid Vibrio cholerae/classification,genetics

Chemicals

DNA Probes DNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yam W C

Department of Microbiology, University of Hong Kong.

Lung M L

Ng K Y

Ng M H

References (12)

12 references, click to expand

Cholera infection and toxin in the rabbit ileal loop.
J Infect Dis. 1966 Apr;116(2):183-90 PMID: 5910273
Polynucleotide sequence relationships among members of Enterobacteriaceae.
J Bacteriol. 1969 May;98(2):637-50 PMID: 4891264
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Nucleotide sequence homology between the heat-labile enterotoxin gene of Escherichia coli and Vibrio cholerae deoxyribonucleic acid.
J Bacteriol. 1980 Oct;144(1):444-6 PMID: 6998963
Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
J Infect Dis. 1980 Dec;142(6):892-8 PMID: 7007526
Clonal origin, restricted natural distribution, and conservation of virulence factors in isolates of enterotoxigenic Escherichia coli serogroup O126.
J Clin Microbiol. 1988 Aug;26(8):1477-81 PMID: 3049655
Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12.
Proc Natl Acad Sci U S A. 1982 May;79(9):2976-80 PMID: 7045877
Molecular epidemiology of Vibrio cholerae in the U.S. Gulf Coast.
J Clin Microbiol. 1982 Jul;16(1):129-34 PMID: 7107852
Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin.
J Biol Chem. 1983 Nov 25;258(22):13722-6 PMID: 6315707
Non-O1 Vibrio cholerae in Thailand: homology with cloned cholera toxin genes.
J Clin Microbiol. 1985 Feb;21(2):288-9 PMID: 3973005
Persistence of cholera in the United States: isolation of Vibrio cholerae O1 from a patient with diarrhea in Maryland.
J Clin Microbiol. 1986 Mar;23(3):624-6 PMID: 3958151
Molecular characterization of environmental and nontoxigenic strains of Vibrio cholerae.
Infect Immun. 1981 May;32(2):661-7 PMID: 7251142

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.27.8.1900-1902.1989

Published

1989-08-00

Pages

1900-2

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC267697

Subset

External Links

PubMed source DOI: 10.1128/jcm.27.8.1900-1902.1989 PMC Full Text View journal details

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