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PMID: 7479858 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 23 ·1995-11-07 ·Pages 10648-52

Smith TJ, Cheng RH, Olson NH, Peterson P, Chase E, Kuhn RJ, Baker TS

Abstract

The structures of Sindbis virus and Ross River virus complexed with Fab fragments from monoclonal antibodies have been determined from cryoelectron micrographs. Both antibodies chosen for this study bind to regions of the virions that have been implicated in cell-receptor recognition and recognize epitopes on the E2 glycoprotein. The two structures show that the Fab fragments bind to the outermost tip of the trimeric envelope spike protein. Hence, the same region of both the Sindbis virus and Ross River virus envelope spike is composed of E2 and is involved in recognition of the cellular receptor.

MeSH Terms
Antibodies, Monoclonal/ultrastructure Antibodies, Viral/ultrastructure Binding Sites Capsid/isolation & purification Capsid Proteins Cryopreservation Image Processing, Computer-Assisted Immunoglobulin Fab Fragments/ultrastructure Microscopy, Immunoelectron Receptors, Virus Ross River virus/ultrastructure Sindbis Virus/ultrastructure Viral Envelope Proteins/isolation & purification
Chemicals
Antibodies, Monoclonal Antibodies, Viral Capsid Proteins E2 protein, Ross River virus Immunoglobulin Fab Fragments Receptors, Virus Viral Envelope Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Smith T J
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
Cheng R H
Olson N H
Peterson P
Chase E
Kuhn R J
Baker T S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.23.10648
Published
1995-11-07
Pages
10648-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40669
Subset
IM
Grants
NIAID NIH HHS · AI33982 · United States
NIGMS NIH HHS · GM33050 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.23.10648 PMC Full Text View journal details
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