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PMID: 7479794 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 22 ·1995-10-24 ·Pages 10408-12

Wheeler JC, Bieschke ET, Tower J

Abstract

Induction of Drosophila hsp70 protein was detected during aging in flight muscle and leg muscle in the absence of heat shock, using an hsp70-specific monoclonal antibody, and in transgenic flies containing hsp70-beta-galactosidase fusion protein reporter constructs. While hsp70 and reporter proteins were induced during aging, hsp70 message levels were not, indicating that aging-specific induction is primarily posttranscriptional. In contrast, hsp22 and hsp23 were found to be induced during aging at the RNA level and with a broader tissue distribution. The same muscle-specific hsp70 reporter expression pattern was observed in young flies mutant for catalase (H2O2:H2O2 oxidoreductase, EC 1.11.1.6). In catalase (cat) hypomorphic lines where flies survived to older ages, the time course of hsp70 reporter expression during aging was accelerated, and the initial and ultimate levels of expression were increased. The hsp70 reporter was also induced in young flies mutant for copper/zinc superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1). Taken together, the results suggest that aging-specific hsp70 expression may be a result of oxidative damage.

MeSH Terms
Aging Animals Base Sequence Blotting, Western DNA Primers Drosophila melanogaster/genetics,physiology Electrophoresis, Polyacrylamide Gel Gene Expression HSP70 Heat-Shock Proteins/biosynthesis,genetics,isolation & purification In Situ Hybridization Molecular Sequence Data Muscles/physiology Oxidative Stress Polymerase Chain Reaction Recombinant Fusion Proteins/biosynthesis beta-Galactosidase/biosynthesis
Chemicals
DNA Primers HSP70 Heat-Shock Proteins Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wheeler J C
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1340, USA.
Bieschke E T
Tower J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.22.10408
Published
1995-10-24
Pages
10408-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40806
Subset
IM
Grants
NIA NIH HHS · AG11833 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.22.10408 PMC Full Text View journal details
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