Home LiteratureArticle Details
PMID: 7479078 Published · ppublish English Journal Article

COUP-TF II homodimers are formed in preference to heterodimers with RXR alpha or TR beta in intact cells.

Nucleic acids research ·Vol. 23 ·No. 20 ·1995-10-25 ·Pages 4143-50

Butler AJ, Parker MG

Abstract

Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) represses the transcriptional activity of a number of nuclear receptors, including that of retinoid receptors (RAR and RXR) and thyroid hormone receptors (TR). Since COUP-TF is capable of binding to DNA in vitro either as a homodimer or as a heterodimer with RXR or TR, it has not been possible to distinguish between competitive DNA binding and heterodimer formation as a mechanism to account for the repression. Using a two-hybrid system we have investigated the dimerisation properties of COUP-TF II in intact cells. In conditions where COUP-TF II homodimers and RXR alpha-RAR alpha heterodimers were formed we were unable to detect the formation of heterodimers between COUP-TF II and RXR alpha. Moreover, we were unable to detect an interaction between COUP-TF II and RXR alpha on DNA. Similarly COUP-TF II homodimers and RXR alpha-TR beta heterodimers are favoured over COUP-TF II-TR beta heterodimers. We conclude that the formation of functionally inactive heterodimers is unlikely to represent a general mechanism by which COUP-TF represses the transcriptional activity of nuclear receptors and favour a model in which repression is mediated by COUP-TF homodimers competing for binding to DNA.

MeSH Terms
Animals Base Sequence Binding, Competitive COUP Transcription Factors Cells, Cultured Chick Embryo DNA/metabolism DNA-Binding Proteins Fibroblasts Fungal Proteins/genetics Humans Models, Genetic Molecular Sequence Data Protein Conformation Receptors, Retinoic Acid/genetics,metabolism Receptors, Steroid Receptors, Thyroid Hormone/genetics,metabolism Recombinant Fusion Proteins/biosynthesis,metabolism Repressor Proteins/chemistry,genetics,metabolism Retinoid X Receptors Saccharomyces cerevisiae Proteins Sequence Deletion Transcription Factors/chemistry,genetics,metabolism Transcriptional Activation/drug effects,physiology Transfection Tretinoin/pharmacology
Chemicals
COUP Transcription Factors DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Receptors, Retinoic Acid Receptors, Steroid Receptors, Thyroid Hormone Recombinant Fusion Proteins Repressor Proteins Retinoid X Receptors Saccharomyces cerevisiae Proteins Transcription Factors Tretinoin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Butler A J
Laboratory of Molecular Endocrinology, Imperial Cancer Research Fund, London, UK.
Parker M G
References (44)
44 references, click to expand
  1. Heterodimerization among thyroid hormone receptor, retinoic acid receptor, retinoid X receptor, chicken ovalbumin upstream promoter transcription factor, and an endogenous liver protein.
    Mol Endocrinol. 1992 Sep;6(9):1468-78 PMID: 1331778
  2. Mutually exclusive interactions of two nuclear orphan receptors determine activity of a cyclic adenosine 3',5'-monophosphate-responsive sequence in the bovine CYP17 gene.
    Mol Endocrinol. 1995 Mar;9(3):327-39 PMID: 7776979
  3. The COUP transcription factor (COUP-TF) is directly involved in the regulation of oxytocin gene expression in luteinizing bovine granulosa cells.
    Biochem Biophys Res Commun. 1992 Nov 30;189(1):496-503 PMID: 1449499
  4. Multiple mechanisms of chicken ovalbumin upstream promoter transcription factor-dependent repression of transactivation by the vitamin D, thyroid hormone, and retinoic acid receptors.
    J Biol Chem. 1993 Feb 25;268(6):4152-60 PMID: 8382695
  5. COUP-TF acts as a competitive repressor for estrogen receptor-mediated activation of the mouse lactoferrin gene.
    Mol Cell Biol. 1993 Mar;13(3):1836-46 PMID: 8441416
  6. Functional conservation of vertebrate seven-up related genes in neurogenesis and eye development.
    EMBO J. 1993 Apr;12(4):1403-14 PMID: 8467797
  7. A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells.
    Anal Biochem. 1986 Jul;156(1):251-6 PMID: 3461723
  8. Firefly luciferase gene: structure and expression in mammalian cells.
    Mol Cell Biol. 1987 Feb;7(2):725-37 PMID: 3821727
  9. Transcription activation by the adenovirus E1a protein.
    Nature. 1989 Mar 2;338(6210):39-44 PMID: 2521923
  10. COUP transcription factor is a member of the steroid receptor superfamily.
    Nature. 1989 Jul 13;340(6229):163-6 PMID: 2739739
  11. A vector for expressing GAL4(1-147) fusions in mammalian cells.
    Nucleic Acids Res. 1989 Sep 25;17(18):7539 PMID: 2798115
  12. The Drosophila seven-up gene, a member of the steroid receptor gene superfamily, controls photoreceptor cell fates.
    Cell. 1990 Jan 26;60(2):211-24 PMID: 2105166
  13. Isolation and characterization of human TR3 receptor: a member of steroid receptor superfamily.
    J Steroid Biochem. 1989;34(1-6):391-5 PMID: 2626032
  14. Regulation of the apolipoprotein AI gene by ARP-1, a novel member of the steroid receptor superfamily.
    Science. 1991 Feb 1;251(4993):561-5 PMID: 1899293
  15. Dopamine activation of an orphan of the steroid receptor superfamily.
    Science. 1991 Jun 14;252(5012):1546-8 PMID: 2047861
  16. Triiodothyronine (T3) decreases binding to DNA by T3-receptor homodimers but not receptor-auxiliary protein heterodimers.
    J Biol Chem. 1992 Feb 25;267(6):3565-8 PMID: 1740410
  17. Retinoid X receptor-COUP-TF interactions modulate retinoic acid signaling.
    Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1448-52 PMID: 1311101
  18. Cloning and sequencing of a COUP transcription factor gene expressed in Xenopus embryos.
    Biochim Biophys Acta. 1992 Feb 11;1129(3):331-4 PMID: 1536886
  19. Evolution of the nuclear receptor gene superfamily.
    EMBO J. 1992 Mar;11(3):1003-13 PMID: 1312460
  20. A transferable silencing domain is present in the thyroid hormone receptor, in the v-erbA oncogene product and in the retinoic acid receptor.
    EMBO J. 1992 Mar;11(3):1015-23 PMID: 1347744
  21. Antagonism between apolipoprotein AI regulatory protein 1, Ear3/COUP-TF, and hepatocyte nuclear factor 4 modulates apolipoprotein CIII gene expression in liver and intestinal cells.
    Mol Cell Biol. 1992 Apr;12(4):1708-18 PMID: 1312668
  22. Ear3/COUP-TF binds most tightly to a response element with tandem repeat separated by one nucleotide.
    Biochem Biophys Res Commun. 1992 Mar 16;183(2):492-8 PMID: 1550558
  23. Antiestrogen ICI 164,384 reduces cellular estrogen receptor content by increasing its turnover.
    Proc Natl Acad Sci U S A. 1992 May 1;89(9):4037-41 PMID: 1570330
  24. The COUP-TFs compose a family of functionally related transcription factors.
    Gene Expr. 1991;1(3):207-16 PMID: 1820218
  25. Repression by ARP-1 sensitizes apolipoprotein AI gene responsiveness to RXR alpha and retinoic acid.
    Mol Cell Biol. 1992 Aug;12(8):3380-9 PMID: 1321332
  26. Transcriptional regulation of human apolipoprotein genes ApoB, ApoCIII, and ApoAII by members of the steroid hormone receptor superfamily HNF-4, ARP-1, EAR-2, and EAR-3.
    J Biol Chem. 1992 Aug 5;267(22):15849-60 PMID: 1639815
  27. Chicken ovalbumin upstream promoter transcription factor (COUP-TF) dimers bind to different GGTCA response elements, allowing COUP-TF to repress hormonal induction of the vitamin D3, thyroid hormone, and retinoic acid receptors.
    Mol Cell Biol. 1992 Sep;12(9):4153-63 PMID: 1324415
  28. COUP orphan receptors are negative regulators of retinoic acid response pathways.
    Mol Cell Biol. 1992 Oct;12(10):4666-76 PMID: 1328857
  29. Thyroid hormone alters the DNA binding properties of chicken thyroid hormone receptors alpha and beta.
    Nucleic Acids Res. 1992 Sep 25;20(18):4803-10 PMID: 1408794
  30. Triiodothyronine (T3) differentially affects T3-receptor/retinoic acid receptor and T3-receptor/retinoid X receptor heterodimer binding to DNA.
    J Biol Chem. 1992 Nov 15;267(32):23248-52 PMID: 1331079
  31. Identification of residues in the estrogen receptor that confer differential sensitivity to estrogen and hydroxytamoxifen.
    Mol Endocrinol. 1993 Feb;7(2):232-40 PMID: 8469236
  32. Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) represses transcription from the promoter of the gene for ornithine transcarbamylase in a manner antagonistic to hepatocyte nuclear factor-4 (HNF-4).
    J Biol Chem. 1993 May 25;268(15):11125-33 PMID: 8496174
  33. RARs and RXRs: evidence for two autonomous transactivation functions (AF-1 and AF-2) and heterodimerization in vivo.
    EMBO J. 1993 Jun;12(6):2349-60 PMID: 8389696
  34. Chicken ovalbumin upstream promoter transcription factor (COUP-TF) binds to a peroxisome proliferator-responsive element and antagonizes peroxisome proliferator-mediated signaling.
    J Biol Chem. 1993 Sep 15;268(26):19169-72 PMID: 8396126
  35. Developmental regulation of the orphan receptor COUP-TF II gene in spinal motor neurons.
    Development. 1994 Jan;120(1):25-36 PMID: 8119130
  36. Transcriptional repression of apolipoprotein AI gene expression by orphan receptor ARP-1.
    J Biol Chem. 1994 May 6;269(18):13185-92 PMID: 8175747
  37. Spatiotemporal expression patterns of chicken ovalbumin upstream promoter-transcription factors in the developing mouse central nervous system: evidence for a role in segmental patterning of the diencephalon.
    Proc Natl Acad Sci U S A. 1994 May 10;91(10):4451-5 PMID: 8183930
  38. A pleiotropic element in the medium-chain acyl coenzyme A dehydrogenase gene promoter mediates transcriptional regulation by multiple nuclear receptor transcription factors and defines novel receptor-DNA binding motifs.
    Mol Cell Biol. 1994 Jul;14(7):4360-72 PMID: 8007945
  39. Functional evidence for ligand-dependent dissociation of thyroid hormone and retinoic acid receptors from an inhibitory cellular factor.
    Mol Cell Biol. 1994 Sep;14(9):5756-65 PMID: 8065310
  40. Functional analysis of a transactivation domain in the thyroid hormone beta receptor.
    J Biol Chem. 1994 Dec 9;269(49):31157-61 PMID: 7983057
  41. The orphan receptor hepatic nuclear factor 4 functions as a transcriptional activator for tissue-specific and hypoxia-specific erythropoietin gene expression and is antagonized by EAR3/COUP-TF1.
    Mol Cell Biol. 1995 Apr;15(4):2135-44 PMID: 7891708
  42. A novel pathway for vitamin A signaling mediated by RXR heterodimerization with NGFI-B and NURR1.
    Genes Dev. 1995 Apr 1;9(7):769-82 PMID: 7705655
  43. An evolutionary conserved COUP-TF binding element in a neural-specific gene and COUP-TF expression patterns support a major role for COUP-TF in neural development.
    Mol Endocrinol. 1994 Dec;8(12):1774-88 PMID: 7708064
  44. SpCOUP-TF: a sea urchin member of the steroid/thyroid hormone receptor family.
    Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10568-72 PMID: 1438252
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.20.4143
Published
1995-10-25
Pages
4143-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307356
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.20.4143 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2