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PMID: 7473196 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Imaging of calcium variations in living dendritic spines of cultured rat hippocampal neurons.

The Journal of physiology ·Vol. 486 ( Pt 2) ·1995-07-15 ·Pages 283-95

Segal M

Abstract

1. Cultured rat hippocampal neurons were loaded with the Ca2+ indicator fura-2 through micropipettes and visualized with an inverted microscope equipped with a high power objective and a cooled CCD camera. The responses of dendritic spines and their parent dendrites to stimuli which evoke a rise of [Ca2+]i were monitored. 2. NMDA caused a rapid and transient rise in [Ca2+]i, which was more evident in the spine than in the parent dendrite. The recovery in both compartments had the same time course, and was dependent on normal [Na+]o. 3. Application of alpha-latrotoxin, which causes release of neurotransmitters from terminals, produced a rise of [Ca2+]i in the dendritic spines, more than in their parent dendrites. Prolonged exposure to the drug eliminated the spine/dendrite disparity. 4. The presence of voltage-gated calcium channels in dendritic spines is indicated by the enhanced calcium rise in spines rather than dendrites of cells depolarized by either intracellular current injection or by raising [K+]o. This rise was attenuated by nifedipine or verapamil, both L-type channel blockers. 5. It is suggested that the dendritic spine constitutes an independent calcium compartment that is closely linked to the parent dendrite.

MeSH Terms
Animals Calcium/metabolism Calcium Channels/physiology Cells, Cultured Dendrites/metabolism Electrophysiology Fluorescence Hippocampus/cytology,metabolism N-Methylaspartate/pharmacology Neurons/metabolism Rats Rats, Wistar Receptors, Purinergic P2/metabolism Receptors, Purinergic P2Y2 Spider Venoms/pharmacology
Chemicals
Calcium Channels P2ry2 protein, rat Receptors, Purinergic P2 Receptors, Purinergic P2Y2 Spider Venoms N-Methylaspartate alpha-latrotoxin Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Segal M
Department of Neurobiology, Weizmann Institute, Rehovot, Israel.
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36 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1995.sp020811
Published
1995-07-15
Pages
283-95
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1156520
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1995.sp020811 PMC Full Text View journal details
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