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PMID: 7440260 Published · ppublish English Journal Article

Quantitation of glucose-6-phosphate dehydrogenase activity in cortical fractions of the nephron in sodium-depleted and sodium-loaded rabbits.

Histochemistry ·Vol. 69 ·No. 1 ·1980-00-00 ·Pages 49-59

Nørgaard T

Abstract

Glucose-6-phosphate dehydrogenase activity was measured quantitatively in isolated cortical fractions of the nephron in sodium-depleted and sodium-loaded rabbits. The samples consisted of isolated fractions of macula densa, proximal convoluted tubule, distal convoluted tubule and glomerulus. In sodium-depleted rabbits enzyme activity was identical to that of normal rabbits. In sodium-loaded rabbits a significant decrease in enzyme activity was found in the macula densa and proximal convoluted tubule. However, using conventional histochemical incubation methods semiquantitative estimation of glucose-6-phosphate dehydrogenase showed a slight decrease in enzyme activity in the macula densa and distal convoluted tubule, and a slight increase in the proximal convoluted tubule during sodium-depletion. During sodium-load a pronounced decrease in enzyme activity was seen in the macula densa and distal convoluted tubule. These results show that semiquantitative histochemical evaluation of changes in enzyme activity is less reliable than the more precise quantitative method especially when there are only slight changes in enzyme activity. Only where there were marked changes in histochemical enzyme activity might the results of quantitative and semiquantitative methods be in accord.

MeSH Terms
Animals Diet Glucosephosphate Dehydrogenase/metabolism Histocytochemistry Kidney Glomerulus/enzymology Kidney Tubules, Distal/enzymology Kidney Tubules, Proximal/enzymology Male Nephrons/drug effects,enzymology Rabbits Sodium/administration & dosage
Chemicals
Sodium Glucosephosphate Dehydrogenase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Nørgaard T
References (27)
27 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00508366
Published
1980-00-00
Pages
49-59
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00508366
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