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PMID: 511594 Published · ppublish English Journal Article

Quantitative measurement of glucose-6-phosphate dehydrogenase in cortical fractions of the rabbit nephron.

Histochemistry ·Vol. 63 ·No. 1 ·1979-09-00 ·Pages 103-13

Nørgaard T

Abstract

A quantitative fluorimetric method is described for estimating the activity of glucose-6-phosphate dehydrogenase in isolated fractions of rabbit nephron from the superficial part of the renal cortex: macula densa, proximal convoluted tubule, distal convoluted tubule and glomerulus. The mean activity in the macula densa region was 2.5 X 10(-18) mol/micrometers 3/min, which was about twice the mean activity of the proximal and distal tubular cells and four times that of the glomeruli. As glucose-6-phosphate dehydrogenase is located in the cytoplasm, the average cytoplasmic enzyme activity of the different tubular cells was calculated: macula densa activity was 4.0 X 10(-18) mol/micrometers 3/min whilst proximal tubular cells showed about a third, and distal tubular cells about a quarter of this activity.

MeSH Terms
Animals Glucosephosphate Dehydrogenase/metabolism Histocytochemistry/methods Kidney Glomerulus/enzymology Kidney Tubules, Distal/enzymology Kidney Tubules, Proximal/enzymology Male Nephrons/cytology,enzymology Rabbits
Chemicals
Glucosephosphate Dehydrogenase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Nørgaard T
References (18)
18 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00508015
Published
1979-09-00
Pages
103-13
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00508015
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