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PMID: 7433091 Published · ppublish English Journal Article

Structural organization of a 17 KB segment of the alpha 2 collagen gene: evaluation by R loop mapping.

Nucleic acids research ·Vol. 8 ·No. 10 ·1980-05-24 ·Pages 2241-53

Schafer MP, Boyd CD, Tolstoshev P, Crystal RG

Abstract

A recombinant phage, SpC3, containing a 17 kb genomic DNA insert representing approximately 60% of the 3' portion of the sheep collagen alpha 2 gene, was evaluated by electron microscopic R loop analysis. A minimum of 17 intervening sequences (introns) and 18 alpha 2 coding sequences (exons) were mapped. With the exception of the 850 base pair exon located at the extreme 3' end of the insert, all exons contained 250 base pairs or less. The total length of all the exons in SpC3 was 3,014 base pairs. The length distribution of the 17 introns ranged from 300 to 1600 base pairs; together, all of the introns comprised 14,070 base pairs of SpC3 DNA. Thus, the DNA region required for coding the interspersed 3 kb of alpha 2 collagen genetic information was 5.6 fold longer than the corresponding alpha 2 mRNA coding sequences.

MeSH Terms
Animals Collagen/genetics DNA, Recombinant Genes Methods Microscopy, Electron Nucleic Acid Hybridization RNA, Messenger/genetics Sheep
Chemicals
DNA, Recombinant RNA, Messenger Collagen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schafer M P
Boyd C D
Tolstoshev P
Crystal R G
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.10.2241
Published
1980-05-24
Pages
2241-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324075
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.10.2241 PMC Full Text View journal details
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