The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes.

The Biochemical journal ·Vol. 188 ·No. 1 ·1980-04-15 ·Pages 137-40

Martin BR, Stein JM, Kennedy EL, Doberska CA

Abstract

Irradiation inactivation was used to monitor changes in the state of adenylate cyclase in rat liver plasma membranes in the presence of F-.F- caused a decrease in the target size from 328000 to 237000 at 0 degrees C and from 329000 to 219000 at 30 degrees C. Adenylate cyclase was activated by F- at both 0 degrees C and 30 degrees C. The effect of F- was biphasic, activating up to a concentration of 10mM and inhibiting at higher concentrations. If adenylate cyclase weas maximally activated with glucagon and p[NH]ppG ([beta gamma-imido]GTP) all concentrations of F- were inhibitory. The implications of the results with respect to the mechanism of activation of adenylate cyclase are discussed.

MeSH Terms

Adenylyl Cyclases/metabolism Animals Cell Membrane/drug effects,enzymology,radiation effects Enzyme Activation/drug effects Female Fluorides/pharmacology Glucagon/pharmacology Guanylyl Imidodiphosphate/pharmacology In Vitro Techniques Liver/drug effects,enzymology,radiation effects Macromolecular Substances Rats Sodium Fluoride/pharmacology Temperature

Chemicals

Macromolecular Substances Guanylyl Imidodiphosphate Sodium Fluoride Glucagon Adenylyl Cyclases Fluorides

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Martin B R

Stein J M

Kennedy E L

Doberska C A

References (9)

9 references, click to expand

Membrane enzyme systems. Molecular size determinations by radiation inactivation.
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The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. I. Properties.
J Biol Chem. 1971 Mar 25;246(6):1849-56 PMID: 4993961
The glucagon-sensitive adenylate cyclase system in plasma membranes of rat liver. VII. Hormonal stimulation: reversibility and dependence on concentration of free hormone.
J Biol Chem. 1972 Apr 10;247(7):2038-43 PMID: 5016642
A highly sensitive adenylate cyclase assay.
Anal Biochem. 1974 Apr;58(2):541-8 PMID: 4827395
Transient complexes. A new structural model for the activation of adenylate cyclase by hormone receptors (guanine nucleotides/irradiation inactivation).
Biochem J. 1979 Nov 15;184(2):253-60 PMID: 230831
The glucagon receptor of rat liver plasma membrane can couple to adenylate cyclase without activating it.
Biochim Biophys Acta. 1976 Jun 17;436(2):489-94 PMID: 179598
A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.
Biochem J. 1977 Mar 1;161(3):555-9 PMID: 851430
Changes in the form of Arrhenius plots of the activity of glucagon-stimulated adenylate cyclase and other hamster liver plasma-membrane enzymes occurring on hibernation.
Biochem J. 1978 Sep 15;174(3):909-19 PMID: 728095
Effects of glucagon on cyclic AMP and carbohydrate metabolism in livers from diabetic rats.
Biochim Biophys Acta. 1974 Mar 20;343(1):250-67 PMID: 4364131

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1880137

Published

1980-04-15

Pages

137-40

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1162547

Subset

External Links

PubMed source DOI: 10.1042/bj1880137 PMC Full Text View journal details

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The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes.

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