A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.

The Biochemical journal ·Vol. 161 ·No. 3 ·1977-03-01 ·Pages 555-9

Martin BR, Voorheis HP

Abstract

A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the reaction catalysed by adenylate cyclase.

MeSH Terms

Adenosine Kinase Adenosine Triphosphate/biosynthesis Adenylate Kinase Isotope Labeling Methods Phosphorus Radioisotopes

Chemicals

Phosphorus Radioisotopes Adenosine Triphosphate Adenosine Kinase Adenylate Kinase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Martin B R

Voorheis H P

References (8)

8 references, click to expand

A spectrophotometric method for the determination of creatine phosphokinase and myokinase.
Biochem J. 1955 Sep;61(1):116-22 PMID: 13260184
Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside.
J Biol Chem. 1951 Dec;193(2):481-95 PMID: 14907737
A highly sensitive adenylate cyclase assay.
Anal Biochem. 1974 Apr;58(2):541-8 PMID: 4827395
Effects of glucagon on cyclic AMP and carbohydrate metabolism in livers from diabetic rats.
Biochim Biophys Acta. 1974 Mar 20;343(1):250-67 PMID: 4364131
Role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones: evidence for multisite transition states.
Adv Cyclic Nucleotide Res. 1975;5:3-29 PMID: 236641
Some properties of adenosine kinase from Ehrlich ascites-tumour cells.
Biochem J. 1968 Jan;106(2):549-55 PMID: 5688928
Modified procedure for the synthesis of 32P-labelled ribonucleoside 5'-monophosphates of high specific activity.
Biochim Biophys Acta. 1968 Feb 26;155(2):609-10 PMID: 5637074
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1610555

Published

1977-03-01

Pages

555-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164541

Subset

External Links

PubMed source DOI: 10.1042/bj1610555 PMC Full Text View journal details

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A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.

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