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PMID: 7371461 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of a BrdU-sensitive site in the cactus mouse (Peromyscus eremicus): chromosomal breakage and sister-chromatid exchange.

Chromosoma ·Vol. 77 ·No. 3 ·1980-00-00 ·Pages 379-89

Schneider NR, Chaganti RS, German J

Abstract

When the thymidine analog BrdU was incorporated into the DNA of a fibroblast cell line derived from the cactus mouse Peromyscus eremicus, a chromosome region with an increased frequency of gaps and breaks was observed. Nearly a third of the chromatid aberrations found at this site were associated with a sister-chromatid exchange (SCE) although this chromosome region showed no increase in sister-chromatid exchange in the absence of a gap or break. SCEs were significantly decreased in the remainder of the chromosome arm when it contained an aberration at the unstable site. This BrdU-sensitive region, unlike others reported, was found not to be late-replicating.--In this chromosome complement, the frequency of sister-chromatid exchange in C-band positive regions was significantly lower than that in C-band negative regions.

MeSH Terms
Animals Bromodeoxyuridine Cell Line Chromosome Aberrations Chromosomes/ultrastructure Crossing Over, Genetic Karyotyping Mice Mitosis Peromyscus/genetics Sister Chromatid Exchange
Chemicals
Bromodeoxyuridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schneider N R
Chaganti R S
German J
References (24)
24 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00286062
Published
1980-00-00
Pages
379-89
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00286062 View journal details
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