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PMID: 7328113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Erythrocyte membrane protein band 3: its biosynthesis and incorporation into membranes.

The Journal of cell biology ·Vol. 91 ·No. 3 Pt 1 ·1981-12-00 ·Pages 637-46

Sabban E, Marchesi V, Adesnik M, Sabatini DD

Abstract

Band 3, a transmembrane protein that provides the anion channel of the erythrocyte plasma membrane, crosses the membrane more than once and has a large amino terminal segment exposes on the cytoplasmic side of the membrane. The biosynthesis of band 3 and the process of its incorporation into membranes were studied in vivo in erythroid spleen cells of anemic mice and in vitro in protein synthesizing cell-free systems programmed with polysomes and messenger RNA (mRNA). In intact cells newly synthesized band 3 is rapidly incorporated into intracellular membranes where it is glycosylated and it is subsequently transferred to the plasma membrane where it becomes sensitive to digestion by exogenous chymotrypsin. The appearance of band 3 in the cell surface is not contingent upon its glycosylation because it proceeds efficiently in cells treated with tunicamycin. The site of synthesis of band 3 in bound polysomes was established directly by in vitro translation experiments with purified polysomes or with mRNA extracted from them. The band-3 polypeptide synthesized in an mRNA-dependent system had the same electrophoretic mobility as that synthesized in cells treated with tunicamycin. When microsomal membranes were present during translation, the in vitro synthesized band-3 polypeptide was cotranslationally glycosylated and inserted into the membranes. This was inferred from the facts that when synthesis was carried out in the presence of membranes the product had a lower electrophoretic mobility and showed partial resistance to protease digestion. Our observations indicate that the primary translation product of band-3 mRNA is not proteolytically processed either co- or posttranslationally. It is, therefore, proposed that the incorporation of band 3 into the endoplasmic reticulum (ER) membrane is initiated by a permanent insertion signal. To account for the cytoplasmic exposure of the amino terminus of the polypeptide we suggest that this signal is located within the interior of the polypeptide. a mechanism that explains the final transmembrane disposition of band 3 in the plasma membrane as resulting from the mode of its incorporation into the ER is presented.

MeSH Terms
Animals Anion Exchange Protein 1, Erythrocyte Blood Proteins/biosynthesis Cells, Cultured Endoplasmic Reticulum/metabolism Erythrocyte Membrane/metabolism Erythrocytes/metabolism Glycoproteins/biosynthesis Kinetics Membrane Proteins/biosynthesis Mice Molecular Weight Protein Biosynthesis Protein Precursors/metabolism Ribosomes/metabolism Spleen Tunicamycin/pharmacology
Chemicals
Anion Exchange Protein 1, Erythrocyte Blood Proteins Glycoproteins Membrane Proteins Protein Precursors Tunicamycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sabban E
Marchesi V
Adesnik M
Sabatini D D
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61 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.91.3.637
Published
1981-12-00
Pages
637-46
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2112817
Subset
IM
Grants
NIA NIH HHS · AG 00378 · United States
NIA NIH HHS · AG 01461 · United States
NIGMS NIH HHS · GM20277 · United States
External Links
PubMed source DOI: 10.1083/jcb.91.3.637 PMC Full Text View journal details
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