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PMID: 831278 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads.

Science (New York, N.Y.) ·Vol. 195 ·No. 4275 ·1977-01-21 ·Pages 302-4

Jacobson BS, Branton D

Abstract

Erythrocytes were ionically attached to polylysine-coated beads 30 micrometers in diameter. The binding of the cells was so tenacious that lysis or disruption of the attached cells left the beads covered by plasma membranes whose cytoplasmic surface was exposed and accessible for further analysis.

MeSH Terms

Cell Fractionation Cell Membrane/physiology,ultrastructure Erythrocyte Membrane/analysis,physiology,ultrastructure Erythrocytes/ultrastructure Glass Humans Membrane Lipids/analysis Membrane Proteins/analysis Methods Phospholipids/analysis

Chemicals

Membrane Lipids Membrane Proteins Phospholipids

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jacobson B S

Branton D

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.831278

Published

1977-01-21

Pages

302-4

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.831278 View journal details

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Plasma membrane: rapid isolation and exposure of the cytoplasmic surface by use of positively charged beads.

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