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PMID: 7287174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Plasmids in Yersinia enterocolitica serotypes O:3 and O:9: correlation with epithelial cell adherence in vitro.

Infection and immunity ·Vol. 33 ·No. 3 ·1981-09-00 ·Pages 870-6

Vesikari T, Nurmi T, Mäki M, Skurnik M, Sundqvist C, Granfors K, Grönroos P

Abstract

Human isolates of Yersinia enterocolitica serotypes O:3 (biotype 4) and O:9 (biotype 3) harbored plasmids sized approximately 47 and 44 megadaltons, respectively. No such plasmids were found in "apathogenic" strains of Y. enterocolitica belonging to biotype 1. There was a positive correlation among the presence of plasmid, autoagglutination, and adherence to and toxicity for HEp-2 cell cultures; all of these properties were lost by culturing at 37 degrees C in the absence of calcium. Strains of Y. enterocolitica O:3 and O:9 cured of the plasmids showed increased invasiveness in the HEp-2 cell culture model, but no invasiveness in guinea pig eye. It is suggested that the plasmids of Y. enterocolitica primarily determine epithelial cell adherence, but may also be associated with other pathogenic properties.

MeSH Terms
Adhesiveness Agglutination Calcium/pharmacology Cell Line Humans Plasmids Serotyping Temperature Yersinia/classification,genetics,pathogenicity
Chemicals
Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Vesikari T
Nurmi T
Mäki M
Skurnik M
Sundqvist C
Granfors K
Grönroos P
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.33.3.870-876.1981
Published
1981-09-00
Pages
870-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350792
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.33.3.870-876.1981 PMC Full Text View journal details
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