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PMID: 7217019 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation of Streptococcus sanguis with monomeric pVA736 plasmid deoxyribonucleic acid.

Journal of bacteriology ·Vol. 146 ·No. 2 ·1981-05-00 ·Pages 826-30

Macrina FL, Jones KR, Welch RA

Abstract

Monomeric and oligomeric forms of a 5.0 x 10(6)-dalton plasmid (conferring erythromycin resistance) were able to genetically transform naturally competent Streptococcus sanguis. Transformation with electrophoretically purified monomer was a second-order process, whereas transformation with a dye-buoyant density gradient-purified plasmid preparation followed one-hit kinetics.

MeSH Terms
Centrifugation, Density Gradient DNA, Bacterial Electrophoresis, Agar Gel Erythromycin/pharmacology Kinetics Plasmids Streptococcus sanguis/genetics Transformation, Bacterial
Chemicals
DNA, Bacterial Erythromycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Macrina F L
Jones K R
Welch R A
References (8)
8 references, click to expand
  1. Survey of the extrachromosomal gene pool of Streptococcus mutans.
    Infect Immun. 1977 Jul;17(1):215-26 PMID: 885614
  2. Evidence for a disseminated plasmid in Streptococcus mutans.
    Infect Immun. 1978 Apr;20(1):296-302 PMID: 669797
  3. The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis.
    Mol Gen Genet. 1978 Nov 9;166(3):259-67 PMID: 105241
  4. Different specific activities of the monomeric and oligomeric forms of plasmid DNA in transformation of B. subtilis and E. coli.
    Mol Gen Genet. 1979 Jul 24;174(3):281-6 PMID: 113646
  5. Molecular cloning of heterologous chromosomal DNA by recombination between a plasmid vector and a homologous resident plasmid in Bacillus subtilis.
    Mol Gen Genet. 1980 Feb;177(3):459-67 PMID: 6246400
  6. Chimeric streptococcal plasmids and their use as molecular cloning vehicles in Streptococcus sanguis (Challis).
    J Bacteriol. 1980 Sep;143(3):1425-35 PMID: 6251030
  7. Properties and transforming activities of two plasmids in Streptococcus pneumoniae.
    Mol Gen Genet. 1980;180(3):573-8 PMID: 7007819
  8. Molecular characterization of unique deletion mutants of the streptococcal plasmid, pAM beta 1.
    Plasmid. 1980 Jul;4(1):8-16 PMID: 6100905
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.146.2.826-830.1981
Published
1981-05-00
Pages
826-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217034
Subset
IM
Grants
NIDDK NIH HHS · R01 DK063250 · United States
NIAID NIH HHS · AI 00382 · United States
NIDCR NIH HHS · DE04224 · United States
NIDCR NIH HHS · KO4 DE00081 · United States
External Links
PubMed source DOI: 10.1128/jb.146.2.826-830.1981 PMC Full Text View journal details
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