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PMID: 7199 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cultivation of mycoplasmas in a modified tissue culture medium.

Applied and environmental microbiology ·Vol. 31 ·No. 6 ·1976-06-00 ·Pages 986-9

Gabridge MG, Singer SE, Esposito RA

Abstract

A new medium, which contained a chemically defined tissue culture base ("medium 199"), was developed for the cultivation of mycoplasmas. When supplemented with albumin, glucose, serum, and yeast extract, the new medium adequately supported the growth of Mycoplasma and Acholeplasma species.

MeSH Terms

Acholeplasma laidlawii/analysis,growth & development Bacterial Proteins/analysis Culture Media Hydrogen-Ion Concentration Mycoplasma/analysis,growth & development

Chemicals

Bacterial Proteins Culture Media

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gabridge M G

Singer S E

Esposito R A

References (16)

16 references, click to expand

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ROLE OF PROTEIN AND PHOSPHOLIPID IN THE GROWTH OF PLEUROPNEUMONIA-LIKE ORGANISMS.
J Bacteriol. 1960 Dec;80(6):851-60 PMID: 16561881
The identification of prereplicative bacteriophage T4 proteins.
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Alterations in the metabolism of hamster tracheas in organ culture after infection by virulent Mycoplasma pneumoniae.
Infect Immun. 1975 Apr;11(4):704-10 PMID: 1120610
Effect of Medium on H(2)O(2) Levels and Peroxidase-Like Activity by Mycoplasma pneumoniae.
Infect Immun. 1971 Jan;3(1):80-6 PMID: 16557950
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.31.6.986-989.1976

Published

1976-06-00

Pages

986-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC169865

Subset

External Links

PubMed source DOI: 10.1128/aem.31.6.986-989.1976 PMC Full Text View journal details

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Cultivation of mycoplasmas in a modified tissue culture medium.

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