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PMID: 4550663 Published · ppublish English Journal Article

Nuclease activities of Mycoplasma gallisepticum as a function of culture age in different media.

Journal of bacteriology ·Vol. 109 ·No. 1 ·1972-01-00 ·Pages 21-4

Cowen BS, Smith SC

Abstract

Levels of deoxyribonuclease and ribonuclease activities in the supernatant (soluble plus ribosomes) fraction of Mycoplasma gallisepticum were assayed and found to be a function of strain, nutrient, and culture age. In yeast hydrolysate-enriched broth, maximal nuclease activities occurred during exponential growth.

MeSH Terms
Animals Bacteriological Techniques Cell Membrane/enzymology Cell-Free System Culture Media Cytoplasm/enzymology DNA, Bacterial/analysis Deoxyribonucleases/metabolism Glucose Horses Hydrogen-Ion Concentration Immune Sera Lactates Mycoplasma/analysis,enzymology,growth & development Penicillins Phenolphthaleins RNA, Bacterial/analysis Ribonucleases/metabolism Ribosomes/enzymology Saccharomyces Species Specificity Time Factors
Chemicals
Culture Media DNA, Bacterial Immune Sera Lactates Penicillins Phenolphthaleins RNA, Bacterial Deoxyribonucleases Ribonucleases Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cowen B S
Smith S C
References (12)
12 references, click to expand
  1. Periodic increase in deoxyribonuclease activity during the cell cycle in synchronized euglena.
    J Cell Biol. 1970 Sep;46(3):613-7 PMID: 5527241
  2. Lability of host-cell DNA in growing cell cultures due to Mycoplasma.
    Science. 1965 Sep 3;149(3688):1098-9 PMID: 5826522
  3. Mycoplasmal deoxyribonuclease activity in virus-infected L-cell cultures.
    J Virol. 1969 Mar;3(3):313-7 PMID: 5782042
  4. Effect of culture medium on antigenic structure of mycoplasma.
    Avian Dis. 1966 May;10(2):173-6 PMID: 5963855
  5. IRREGULAR VARIATION IN CELLULAR NITROGEN ASSOCIATED WITH CULTURE AGE OF MYCOPLASMA GALLISEPTICUM.
    J Bacteriol. 1963 Oct;86:880-1 PMID: 14066489
  6. The purification and properties of micrococcal nuclease.
    J Biol Chem. 1961 Nov;236:3014-9 PMID: 13860347
  7. Counts of virus particles by sedimentation on agar and electron micrography.
    Proc Soc Exp Biol Med. 1952 Oct;81(1):75-9 PMID: 13047318
  8. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. VI. CHANGES IN ENZYME LEVELS IN RESPONSE TO ALTERATIONS IN PHYSIOLOGICAL STATE.
    J Biol Chem. 1964 Sep;239:2964-74 PMID: 14217883
  9. Physiological and serologic comparisons of PPLO from various sources.
    Ann N Y Acad Sci. 1960 Jan 15;79:521-30 PMID: 13839052
  10. Localization of Enzymes in Mycoplasma.
    J Bacteriol. 1965 Sep;90(3):617-22 PMID: 16562057
  11. Nucleic acid content of pleuropneumonialike organisms from human sources.
    J Bacteriol. 1957 Dec;74(6):811-7 PMID: 13502310
  12. NUCLEASES OF MYCOPLASMA.
    J Gen Microbiol. 1964 Aug;36:323-32 PMID: 14195654
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.109.1.21-24.1972
Published
1972-01-00
Pages
21-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247246
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.109.1.21-24.1972 PMC Full Text View journal details
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