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PMID: 7155897 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Organization and nucleotide sequence of nuclear 5S rRNA genes in yellow lupin (Lupinus luteus).

Nucleic acids research ·Vol. 10 ·No. 23 ·1982-12-11 ·Pages 7635-42

Rafalski JA, Wiewiorowski M, Söll D

Abstract

Genomic blots of yellow lupin (Lupinus luteus) DNA digested with restriction nucleases and probed with 32P-labelled Lupinus 5S RNA reveal that 5S DNA is organized as tandemly repeated sequences of one size class, 342 bp. The DNA is extensively methylated. Two cloned BamHI ribosomal repeats were sequenced, revealing sequence divergence within both the coding and spacer regions.

MeSH Terms
Base Composition Base Sequence Cloning, Molecular DNA/genetics,isolation & purification DNA, Ribosomal Fabaceae/genetics Genes Molecular Weight Nucleic Acid Conformation Nucleic Acid Hybridization Plants/genetics Plants, Medicinal Plasmids RNA, Ribosomal/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rafalski J A
Wiewiorowski M
Söll D
References (23)
23 references, click to expand
  1. Nucleotide sequence analysis of the cytoplasmic 5S ribosomal ribonucleic acid from five species of flowering plants.
    Biochem J. 1973 Dec;135(4):845-51 PMID: 4778279
  2. Studies on human tRNA. I. The rapid, large scale isolation and partial fractionation of placenta and liver tRNA.
    Nucleic Acids Res. 1975 Jan;2(1):21-42 PMID: 236551
  3. Evidence for the nucleotide sequence of 5-S rRNA from the flowering plant Secale cereale (Rye).
    Eur J Biochem. 1976 Dec;71(1):33-8 PMID: 1009953
  4. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  5. Nucleotide sequence of tRNAPhe from the seeds of lupin (Lupinus luteus). Comparison of the major species with wheat germ tRNAPhe.
    Acta Biochim Pol. 1977;24(4):301-18 PMID: 264239
  6. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  7. Evolutionary change in 5S RNA secondary structure and a phylogenic tree of 54 5S RNA species.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):381-5 PMID: 284354
  8. Conservation of the structures of plant tRNAs and aminoacyl-tRNA synthetases.
    FEBS Lett. 1979 Jun 1;102(1):194-7 PMID: 456588
  9. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease I of Bacillus amyloliquefaciens H.
    J Mol Biol. 1979 Sep 15;133(2):289-94 PMID: 231662
  10. Cloning and characterization of ribosomal RNA genes from wheat and barley.
    Nucleic Acids Res. 1979 Dec 11;7(7):1869-85 PMID: 537913
  11. Gel electrophoresis of restriction fragments.
    Methods Enzymol. 1979;68:152-76 PMID: 232210
  12. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
  13. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  14. Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes.
    Nucleic Acids Res. 1980 Nov 11;8(21):4851-65 PMID: 7443527
  15. A split promoter for a eucaryotic tRNA gene.
    Cell. 1981 May;24(2):573-85 PMID: 7237560
  16. The nucleotide sequence of spinach cytoplasmic 5 S ribosomal RNA.
    J Biol Chem. 1981 Jul 25;256(14):7515-7 PMID: 7251607
  17. Localization of the 5S rRNA genes and evidence for diversity in the 5S rDNA region of maize.
    Gene. 1981 Oct;15(1):7-20 PMID: 6170540
  18. Organisation of the 5S RNA genes in flax.
    Nucleic Acids Res. 1981 Nov 25;9(22):5895-904 PMID: 6171777
  19. Internal control regions for transcription of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6657-61 PMID: 6947245
  20. Transcription of Xenopus 5S ribosomal RNA genes.
    Nature. 1982 Jan 14;295(5845):101-5 PMID: 7057877
  21. Collection of published 5S and 5.8S RNA sequences and their precursors.
    Nucleic Acids Res. 1982 Jan 22;10(2):r93-115 PMID: 6174939
  22. Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1195-9 PMID: 6951168
  23. Sequence variation and methylation of the flax 5S RNA genes.
    Nucleic Acids Res. 1982 Aug 11;10(15):4501-14 PMID: 6290983
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.23.7635
Published
1982-12-11
Pages
7635-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327035
Subset
IM
Databases
GENBANK
M13617, M13618
External Links
PubMed source DOI: 10.1093/nar/10.23.7635 PMC Full Text View journal details
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