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PMID: 7155802 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mechanical characteristics of chemically skinned guinea-pig taenia coli.

Pflugers Archiv : European journal of physiology ·Vol. 395 ·No. 4 ·1982-12-00 ·Pages 277-84

Arner A

Abstract

Strips of intact and chemically skinned (Triton X-100) taenia coli were mounted for isometric and quick-release experiments at 23 degrees C. Active force increased in repeated high-K+ induced contractures in the intact muscle. Stable maximal force was 313 +/- 24 mN/mm2 (n = 6). The skinned preparations activated by Ca2+, at 2 mM Mg2+, 3.2 mM MgATP and ionic strength 0.085 M, gave half maximal force at pCa = 5.62 +/- 0.4 and a maximal force (63 +/- 8 mN/mm2) at pCa = 4.5 (20-25% of the control K+-responses prior to skinning but about 60% of the first K+-response). Force-velocity relations were obtained from intact muscles and from the same muscles chemically skinned and activated at optimal Ca2+. Maximal shortening velocity (Vmax) was unaltered in the skinned preparation compared to the intact muscle (0.138 +/- 0.011 vs 0.140 +/- 0.006 L/s) indicating similar kinetics of actomyosin interaction. In the intact muscle a decrease in Vmax was found when the Ca2+ concentration was reduced. Calmodulin (1 microM) increased Ca2+ sensitivity (by about 0.6 log units) of the skinned preparation but at optimal Ca2+ caused no alteration in isometric force or Vmax.

MeSH Terms
Animals Biomechanical Phenomena Calcium/physiology Cell Membrane/drug effects Colon/physiology Female Guinea Pigs Male Muscle Contraction Muscle, Smooth/physiology
Chemicals
Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Arner A
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35 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00580790
Published
1982-12-00
Pages
277-84
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00580790 View journal details
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