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PMID: 7145700 Published · ppublish English Journal Article

A yeast transcription system for the 5S rRNA gene.

Nucleic acids research ·Vol. 10 ·No. 17 ·1982-09-11 ·Pages 5223-38

van Keulen H, Thomas DY

Abstract

A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size and oligonucleotide composition as in vivo 5S rRNA. The in vitro transcription extract does not transcribe yeast tRNA genes. The extract does increase the transcription of tRNA genes packaged in chromatin.

MeSH Terms
Cell Nucleus/metabolism Cell-Free System Cloning, Molecular Genes Kinetics Protein Biosynthesis RNA, Ribosomal/genetics RNA, Transfer/genetics Saccharomyces/genetics Transcription, Genetic
Chemicals
RNA, Ribosomal RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van Keulen H
Thomas D Y
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.17.5223
Published
1982-09-11
Pages
5223-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320867
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.17.5223 PMC Full Text View journal details
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