Maintenance of Laboratory strains of obligately anaerobic rumen bacteria.

Applied and environmental microbiology ·Vol. 44 ·No. 2 ·1982-08-00 ·Pages 499-501

Teather RM

Abstract

Cultures of rumen bacteria can be stored at -20 degrees C for at least 2 years in a liquid medium containing 20% glycerol. Thawing, sampling, and refreezing do not significantly affect viability.

MeSH Terms

Anaerobiosis Animals Bacteria/growth & development Culture Media Freeze Drying Kinetics Rumen/microbiology

Chemicals

Culture Media

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Teather R M

References (5)

5 references, click to expand

A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.
Appl Microbiol. 1974 May;27(5):985-7 PMID: 4598231
Studies on the Nitrogen Requirements of Some Ruminal Cellulolytic Bacteria.
Appl Microbiol. 1961 Mar;9(2):96-103 PMID: 16349603
Some Nutritional Requirements of the Genus Ruminococcus.
Appl Microbiol. 1961 Mar;9(2):91-5 PMID: 16349602
The anaerobic mesophilic cellulolytic bacteria.
Bacteriol Rev. 1950 Mar;14(1):1-49 PMID: 15420122
Use of syringe methods for anaerobiosis.
Am J Clin Nutr. 1972 Dec;25(12):1318-23 PMID: 4565348

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.44.2.499-501.1982

Published

1982-08-00

Pages

499-501

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC242039

Subset

External Links

PubMed source DOI: 10.1128/aem.44.2.499-501.1982 PMC Full Text View journal details

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Maintenance of Laboratory strains of obligately anaerobic rumen bacteria.

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