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PMID: 7106769 Published · ppublish English Journal Article

A simple reproducible method for prometaphase chromosome analysis.

Human genetics ·Vol. 60 ·No. 4 ·1982-00-00 ·Pages 328-33

Rybak J, Tharapel A, Robinett S, Garcia M, Mankinen C, Freeman M

Abstract

A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase.

MeSH Terms
Azure Stains Cell Division Chromosome Banding Chromosomes, Human/ultrastructure Colchicine Culture Media Dactinomycin Humans Hypertonic Solutions Lymphocytes/cytology Metaphase Plasma Prophase
Chemicals
Azure Stains Culture Media Hypertonic Solutions Dactinomycin Colchicine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rybak J
Tharapel A
Robinett S
Garcia M
Mankinen C
Freeman M
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36 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00569213
Published
1982-00-00
Pages
328-33
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00569213 View journal details
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