Specific banding patterns of human chromosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 9 ·1971-09-00 ·Pages 2073-7

Drets ME, Shaw MW

Abstract

Individual pairs of human chromosomes can be reliably identified by a new method that does not require special optical equipment and that results in permanent preparations. This method, which is based on treatment of the chromosomes in situ with NaOH, followed by incubation in sodium chloride-trisodium citrate and Giemsa staining, results in highly specific banding patterns in characteristic regions of the chromosome arms. It should prove useful for the detection of small structural changes in chromosomes.

MeSH Terms

Chromosomes Chromosomes, Human, 1-3 Chromosomes, Human, 13-15 Chromosomes, Human, 16-18 Chromosomes, Human, 19-20 Chromosomes, Human, 21-22 and Y Chromosomes, Human, 4-5 Chromosomes, Human, 6-12 and X Female Fibroblasts/cytology Humans Karyotyping Lymphocytes/cytology Male Methods Sex Chromosomes Sodium Hydroxide Staining and Labeling

Chemicals

Sodium Hydroxide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Drets M E

Shaw M W

References (7)

7 references, click to expand

Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA.
Science. 1966 Nov 11;154(3750):791-4 PMID: 5919450
Chromosomal localization of mouse satellite DNA.
Science. 1970 Jun 12;168(3937):1356-8 PMID: 5462793
Differential binding of alkylating fluorochromes in human chromosomes.
Exp Cell Res. 1970 Jun;60(3):315-9 PMID: 5422961
The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability.
Hereditas. 1972;67(1):89-102 PMID: 4142006
Localization of heterochromatin in human chromosomes.
Cytogenetics. 1971;10(2):81-6 PMID: 4106483
Computerized chromosome identification by aid of the quinacrine mustard fluorescence technique.
Hereditas. 1972;67(1):103-10 PMID: 4142003
Localization of repetitive DNA in the chromosomes of Microtus agrestis by means of in situ hybridization.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.9.2073

Published

1971-09-00

Pages

2073-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389354

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.9.2073 PMC Full Text View journal details

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Specific banding patterns of human chromosomes.

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