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PMID: 7072716 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24.

American journal of human genetics ·Vol. 34 ·No. 2 ·1982-03-00 ·Pages 227-34

Harper ME, Barrera-Saldaña HA, Saunders GF

Abstract

Recombinant plasmid HCS-pBR322 containing a 550-base-pair (bp) insert of cDNA to human placental lactogen (hPL) mRNA was 3H-labeled by nick translation and hybridized in situ to human chromosome preparations in the presence of 10% dextran sulfate. A high percentage of cells (80%) were found to exhibit label on the distal end of the long arm of chromosome 17. Silver grains on this region constituted 25.5% of all labeled sites, allowing assignment of the hPL and growth hormone (hGH) genes, which have over 90% nucleotide homology in their coding sequences, to 17q22-24. A gene copy number experiment showed that both genes are present in approximately three copies per haploid genome.

MeSH Terms
Chromosome Banding Chromosome Mapping Chromosomes, Human, 16-18 DNA Genes Growth Hormone/genetics Humans Hybrid Cells Nucleic Acid Hybridization Placental Lactogen/genetics Plasmids RNA, Messenger
Chemicals
RNA, Messenger Growth Hormone DNA Placental Lactogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harper M E
Barrera-Saldaña H A
Saunders G F
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1982-03-00
Pages
227-34
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1685277
Subset
IM
Grants
NCI NIH HHS · CA-16672 · United States
NCI NIH HHS · CA-20124 · United States
NIGMS NIH HHS · GM-23965 · United States
External Links
PubMed source PMC Full Text View journal details
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