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PMID: 7071018 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of highly purified satellite DNA containing chromatin from the mouse.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1481-94

Zhang XY, Hörz W

Abstract

A purification scheme for satellite DNA containing chromatin from mouse liver has been developed. It is based on the highly condensed state of the satellite chromatin and also takes advantage of its resistance to digestion by certain restriction nucleases. Nuclei are first treated with micrococcal nuclease and the satellite chromatin enriched 3-5 fold by extraction of the digested nuclei under appropriate conditions. Further purification is achieved by digestion of the chromatin with a restriction nuclease that leaves satellite DNA largely intact but degrades non-satellite DNA extensively. In subsequent sucrose gradient centrifugation the rapidly sedimenting chromatin contains more than 70% satellite DNA. This material has the same histone composition as bulk chromatin. No significant differences were detected in an analysis of minor histone variants. Nonhistone proteins are present only in very low amounts in the satellite chromatin fraction, notably the HMG proteins are strongly depleted.

MeSH Terms
Animals Cell Nucleus/analysis Centrifugation, Density Gradient Chromatin/analysis DNA, Satellite/isolation & purification Histones/isolation & purification Liver/analysis Mice Micrococcal Nuclease
Chemicals
Chromatin DNA, Satellite Histones Micrococcal Nuclease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhang X Y
Hörz W
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1481
Published
1982-03-11
Pages
1481-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320544
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.5.1481 PMC Full Text View journal details
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