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PMID: 7060088 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Endocytosis in absorptive cells of cultured human small-intestinal tissue: effect of cytochalasin B and D.

Cell and tissue research ·Vol. 222 ·No. 1 ·1982-00-00 ·Pages 113-26

Blok J, Scheven BA, Mulder-Stapel AA, Ginsel LA, Daems WT

Abstract

The effect of cytochalasin B (CB) and cytochalasin D (CD) on the endocytotic uptake of horseradish peroxidase (HRP) by intestinal absorptive cells was investigated by morphometric methods. The results showed that CD inhibited endocytosis considerably, and without any detrimental side-effects. CB had hardly any effect on the endocytosis of HRP, but caused a significant decrease in the number of apical vesicles and tubules involved in the transport of cell-coat glycoproteins from the Golgi apparatus to the brush border. Electron-microscopic autoradiographic analysis of the effect of CD showed that although endocytosis is inhibited significantly by the drug, the amount of radiolabelled cell-coat material entering the lysosome-like bodies was unaltered compared with control cultures. These observations support our hypothesis that the cell-coat glycoproteins of the absorptive cells enter the lysosome-like bodies by a crinophagic rather than by an exocytotic-endocytotic mechanism.

MeSH Terms
Cells, Cultured Cytochalasin B/pharmacology Cytochalasins/pharmacology Dimethyl Sulfoxide/pharmacology Endocytosis/drug effects Glycoproteins/metabolism Horseradish Peroxidase/metabolism Humans Intestinal Absorption/drug effects Microscopy, Electron
Chemicals
Cytochalasins Glycoproteins Cytochalasin B Horseradish Peroxidase Dimethyl Sulfoxide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blok J
Scheven B A
Mulder-Stapel A A
Ginsel L A
Daems W T
References (30)
30 references, click to expand
  1. The site of incorporation of sialic acid residues into glycoproteins and the subsequent fates of these molecules in various rat and mouse cell types as shown by radioautography after injection of [3H]N-acetylmannosamine. II. Observations in tissues other than liver.
    J Cell Biol. 1981 Jan;88(1):16-28 PMID: 7204485
  2. The effect of chloroquine on lysosomal function and cell-coat glycoprotein transport in the absorptive cells of cultured human small-intestinal tissue.
    Cell Tissue Res. 1981;218(2):227-51 PMID: 7261028
  3. Migration of glycoprotein from the Golgi apparatus to the surface of various cell types as shown by radioautography after labelled fucose injection into rats.
    J Cell Biol. 1974 Jan;60(1):258-84 PMID: 4809242
  4. Endocytosis.
    Annu Rev Biochem. 1977;46:669-722 PMID: 332066
  5. Qualitative and quantitative preservation of the fine structure of absorptive cells in cultured biopsies of human small-intestine.
    Cell Tissue Res. 1977 Jul 11;181(2):143-62 PMID: 880627
  6. Formation of cell coat material for the whole surface of columnar cells in the rat small intestine, as visualized by radioautography with L-fucose-3H.
    J Cell Biol. 1970 Aug;46(2):409-16 PMID: 5449186
  7. Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates.
    Anal Biochem. 1977 Feb;77(2):464-70 PMID: 842829
  8. Cytochalasin B: inhibition of glucose and glucosamine transport.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1430-4 PMID: 4338593
  9. Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B.
    J Biol Chem. 1972 Jun 25;247(12):4102-5 PMID: 4338238
  10. Inhibition of phagocytosis and plasma membrane mobility of the cultivated macrophage by cytochalasin B. Role of subplasmalemmal microfilaments.
    J Cell Biol. 1974 Sep;62(3):647-59 PMID: 4368822
  11. Organ culture of mucosal biopsies of human small intestine.
    J Clin Invest. 1969 Aug;48(8):1423-32 PMID: 5796354
  12. Turnover studies of human intestinal brush border membrane glycoproteins in organ culture.
    Enzyme. 1979;24(2):96-106 PMID: 456341
  13. Inhibition of glycoconjugate secretion by colchicine and cytochalasin B. An in vitro study of human airway.
    Cell Tissue Res. 1981;214(1):107-18 PMID: 7471168
  14. Endocytosis in absorptive cells of cultured human small-intestinal tissue: horseradish peroxidase, lactoperoxidase, and ferritin as markers.
    Cell Tissue Res. 1981;216(1):1-13 PMID: 7226201
  15. Passage of fucose- 3 H label from the Golgi apparatus into dense and multivesicular bodies in the duodenal columnar cells and hepatocytes of the rat.
    J Cell Biol. 1971 Dec;51(3):875-81 PMID: 4331504
  16. Cytochalasin B. Dissociation of pinocytosis and phagocytosis by peritoneal macrophages.
    Exp Cell Res. 1973 Apr;79(1):73-8 PMID: 4798426
  17. Transport of radiolabelled glycoprotein to cell surface and lysosome-like bodies of absorptive cells in clutured small-intestinal tissue from normal subjects and patients with a lysosomal storage disease.
    Virchows Arch B Cell Pathol Incl Mol Pathol. 1979 Jun 29;30(3):245-73 PMID: 43006
  18. The transport of lysosomal enzymes.
    J Supramol Struct. 1977;6(1):95-101 PMID: 895139
  19. Role of contractile microfilaments in macrophage movement and endocytosis.
    Nat New Biol. 1971 Aug 4;232(31):153-5 PMID: 4937093
  20. Inhibition of pinocytosis by cytochalasin B. Decrease in intracellular lysosomal-enzyme activities and increased storage of glycosaminoglycans.
    Eur J Biochem. 1974 Oct 2;48(2):357-63 PMID: 4280968
  21. Fucosidosis and I-cell disease: a fine structural and silver-staining study of abnormal inclusion bodies in small-intestinal cells.
    Virchows Arch B Cell Pathol. 1978 Mar 29;27(2):99-117 PMID: 77075
  22. Biosynthesis of brush border glycoproteins by human small intestinal mucosa in organ culture.
    Biochim Biophys Acta. 1977 Jun 16;467(3):327-39 PMID: 884074
  23. Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling.
    J Cell Biol. 1980 Jun;85(3):839-52 PMID: 7190150
  24. Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):253-7 PMID: 4257818
  25. Migration of glycoprotein from golgi apparatus to cell coat in the columnar cells of the duodenal epithelium.
    J Cell Biol. 1970 Jun;45(3):668-73 PMID: 5459950
  26. Resolution of a gold latensification-elon ascorbic acid developer for Ilford L4 emulsion.
    Histochemistry. 1979 Jul 11;61(3):343-6 PMID: 478993
  27. Endocytosis in Chang liver cells. Quantitation by sucrose- 3 H uptake and inhibition by cytochalasin B.
    J Cell Biol. 1971 Sep;50(3):804-17 PMID: 4329157
  28. Fine structure and silver-staining patterns of lysosome-like bodies in absorptive cells of the small intestine in normal children and children with a lysosomal storage disease.
    Virchows Arch B Cell Pathol. 1973 Jun 25;13(2):119-44 PMID: 4201198
  29. Cytochalasin B binding to Ehrlich ascites tumor cells and its relationship to glucose carrier.
    Biochim Biophys Acta. 1981 Apr 6;642(2):392-404 PMID: 7284364
  30. Purification of the human intestinal brush border membrane.
    Biochim Biophys Acta. 1973 Sep 27;323(1):98-112 PMID: 4356391
Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00218292
Published
1982-00-00
Pages
113-26
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00218292 View journal details
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