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PMID: 7044369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ca2+-uptake properties of two populations of mitochondria from normal and denervated rat soleus muscle.

The Biochemical journal ·Vol. 200 ·No. 3 ·1981-12-15 ·Pages 671-7

Joffe M, Savage N, Isaacs H

Abstract

Ultraturrax- and Nagarse-released populations of mitochondria were characterized with respect to their Ca2+-uptake activities (i) by means of the indirect polarographic technique and (ii) directly by the 45Ca Ruthenium Red-quench method of Reed & Bygrave [(1974) Biochem. J. 140, 143-155]. The denervated-muscle subsarcolemmal and intermyofibrillar mitochondrial fractions displayed markedly decreased rates and capacities for Ca2+ uptake compared with their respective controls. The implications of these findings with respect to the process of cell necrosis are discussed.

MeSH Terms
Animals Biological Transport Calcium/metabolism Female In Vitro Techniques Mitochondria, Muscle/drug effects,metabolism Muscle Denervation Polarography Rats Rats, Inbred Strains Stimulation, Chemical Subtilisins/pharmacology
Chemicals
Subtilisins Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Joffe M
Savage N
Isaacs H
References (21)
21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2000671
Published
1981-12-15
Pages
671-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163590
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2000671 PMC Full Text View journal details
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