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PMID: 704348 Published · ppublish English Journal Article

Nucleotide sequence of the O gene and of the origin of replication in bacteriophage lambda DNA.

Nucleic acids research ·Vol. 5 ·No. 9 ·1978-09-00 ·Pages 3141-56

Scherer G

Abstract

The nucleotide sequence of the O gene in bacteriophage lambda DNA is presented. According to two possible initiator codons, the primary structure of the O protein deduced from the DNA sequence consists of 278 or 299 amino acid residues. Structure and function of the O protein--one of the two phage initiator proteins for lambda DNA replication--are discussed in the light of a secondary structure model for the O protein. The central part of the O gene contains a cluster of symmetrical sequences extending over 160 base pairs. The point mutation of the cis-dominant replication mutant ti12 is located in this region.

MeSH Terms
Amino Acid Sequence Base Sequence Coliphages/genetics DNA Replication DNA Restriction Enzymes/metabolism DNA, Viral/genetics Genes, Viral Molecular Weight Protein Conformation Viral Proteins/genetics Virus Replication
Chemicals
DNA, Viral Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Scherer G
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.9.3141
Published
1978-09-00
Pages
3141-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342237
Subset
IM
Databases
GENBANK
J02459, M17233
External Links
PubMed source DOI: 10.1093/nar/5.9.3141 PMC Full Text View journal details
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