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PMID: 7017707 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Prophage (phi 80) induction in Escherichia coli K-12 by specific deoxyoligonucleotides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 1 ·1981-01-00 ·Pages 138-42

Irbe RM, Morin LM, Oishi M

Abstract

A cell preparation that is permeable to proteins and oligonucleotides yet produces infectious phage particles after induction treatments was obtained by plasmolysis of Escherichia coli cells lysogenic for phi 80. When the permeabilized cells were exposed to specific oligo(deoxynucleotides), prophage (phi 80) was induced during further incubation. Of the dinucleotides tested, only d(A-G), d(G-G), and d(I-G) induced prophage. The essential base sequence of the deoxydinucleotides for the induction was determined to be deoxy(purine-G). Among oligo(deoxynucleotides) with unique base composition examined, only oligo(deoxyguanylates) exhibited the inducing activity. Although this specific oligo(deoxynucleotide)-triggered induction occurred in recB- cells, the induction was not detected in recA- cells or in the cells lysogenic for induction-negative phi 80(ind-). Possible biological significance of the oligo(deoxynucleotide)-triggered prophage induction is discussed.

MeSH Terms
Cell Membrane Permeability Coliphages/metabolism Deoxyribonucleotides/pharmacology Escherichia coli/drug effects,metabolism Viral Plaque Assay Virus Activation/drug effects
Chemicals
Deoxyribonucleotides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Irbe R M
Morin L M
Oishi M
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.1.138
Published
1981-01-00
Pages
138-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319006
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM07308 · United States
NIGMS NIH HHS · GM-21073 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.1.138 PMC Full Text View journal details
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