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PMID: 7012125 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli.

Journal of bacteriology ·Vol. 146 ·No. 1 ·1981-04-00 ·Pages 422-5

Brown S, Brickman ER, Beckwith J

Abstract

We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.

MeSH Terms

Escherichia coli/genetics,isolation & purification Genes Genetic Techniques Mutation

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Brown S

Brickman E R

Beckwith J

References (12)

12 references, click to expand

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Specialized transduction of colicin E1 DNA in Escherichia coli K-12.
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3238-42 PMID: 787989
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Proc Natl Acad Sci U S A. 1977 Mar;74(3):1162-6 PMID: 322147
Transcription termination at the end of the tryptophan operon of Escherichia coli.
J Mol Biol. 1977 May 25;112(3):423-36 PMID: 327077
Amber mutations in Escherichia coli essential genes: isolation of mutants affected in the ribosomes.
Mol Gen Genet. 1977 Nov 29;157(2):149-53 PMID: 340921
Fusions of the lac operon to the transfer RNA gene tyrT of Escherichia coli.
J Mol Biol. 1979 May 25;130(3):285-301 PMID: 381675
Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication.
J Bacteriol. 1979 Dec;140(3):825-34 PMID: 160413
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Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.146.1.422-425.1981

Published

1981-04-00

Pages

422-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217102

Subset

External Links

PubMed source DOI: 10.1128/jb.146.1.422-425.1981 PMC Full Text View journal details

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Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli.

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