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PMID: 7011974 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence that botulinum C2 toxin has two dissimilar components.

Infection and immunity ·Vol. 29 ·No. 2 ·1980-08-00 ·Pages 390-4

Iwasaki M, Ohishi I, Sakaguchi G

Abstract

Botulinum C2 toxin produced by most toxigenic and nontoxigenic strains of Clostridium botulinum types C and D contains two distinct protein components, and these were separated by ion-exchange chromatography and gel filtration. Neither of these components manifested the original toxicity, but the original toxicity was restored when the two components were mixed together and trypsinized. This indicates that C2 toxin consists of two dissimilar protein components and that the cooperation of the two is required to elicit toxicity.

MeSH Terms

Animals Botulinum Toxins/analysis,classification Chemical Phenomena Chemistry Chromatography, Gel Chromatography, Ion Exchange Clostridium botulinum/metabolism Culture Media Lethal Dose 50 Mice

Chemicals

Culture Media Botulinum Toxins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Iwasaki M

Ohishi I

Sakaguchi G

References (16)

16 references, click to expand

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Acid precipitation of Clostridium botulinum type C and D toxins from whole culture by addition of ribonucleic acid as a precipitation aid.
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Clostridium botulinum type D toxin: purification, molecular structure, and some immunological properties.
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Enrichment, isolation, and cultural characteristics of marine strains of Clostridium botulinum type C.
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Activation of a toxic component of Clostridium botulinum types C and D by trypsin.
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Activation of Clostridium botulinum type E toxin by trypsin.
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Sporulation and C2 toxin production by Clostridium botulinum type C strains producing no C1 toxin.
Microbiol Immunol. 1978;22(10):591-6 PMID: 368526
Molecular construction of Clostridium botulinum type A toxins.
Infect Immun. 1975 Dec;12(6):1262-70 PMID: 54335
Separation of toxin and hemagglutinin from crystalline toxin of Clostridium botulinum type A by anion exchange chromatography and determination of their dimensions by gel filtration.
J Biol Chem. 1968 Mar 10;243(5):1065-72 PMID: 4867941
The toxic antigenic factors produced by Clostridium botulinum types C and D.
Onderstepoort J Vet Res. 1971 Jun;38(2):93-8 PMID: 4950122
Tryptic activation of Clostridium botulinum type C beta toxin.
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Distribution of Clostridium botulinum type C in Ishikawa prefecture, and applicability of agglutination to identification of nontoxigenic isolates of C. botulinum type C.
Microbiol Immunol. 1977;21(3):127-36 PMID: 327214
Dissociation and reconstitution of 12-S toxin of Clostridium botulinum type E.
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Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Molecular construction of Clostridium botulinum type F progenitor toxin.
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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.29.2.390-394.1980

Published

1980-08-00

Pages

390-4

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC551130

Subset

External Links

PubMed source DOI: 10.1128/iai.29.2.390-394.1980 PMC Full Text View journal details

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Evidence that botulinum C2 toxin has two dissimilar components.

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