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PMID: 344224 Published · ppublish English Journal Article

Acid precipitation of Clostridium botulinum type C and D toxins from whole culture by addition of ribonucleic acid as a precipitation aid.

Infection and immunity ·Vol. 19 ·No. 2 ·1978-02-00 ·Pages 749-51

Iwasaki M, Sakaguchi G

Abstract

The ratios of ribonucleic acid to protein contents of Clostridium botulinum type C, D, and E cultures were lower than those of type A, B, and F cultures. Addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type C and D toxins, but not that of type E toxin.

MeSH Terms
Bacterial Proteins/analysis Botulinum Toxins/analysis,isolation & purification Chemical Precipitation Clostridium botulinum/analysis RNA RNA, Bacterial/analysis Saccharomyces cerevisiae
Chemicals
Bacterial Proteins RNA, Bacterial RNA Botulinum Toxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Iwasaki M
Sakaguchi G
References (11)
11 references, click to expand
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  9. Studies on immunity to toxins of Clostridium botulinum. VI. Purification and detoxification of type D toxin and the immunological response to toxoid.
    J Bacteriol. 1960 Mar;79:372-8 PMID: 13807649
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    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.19.2.749-751.1978
Published
1978-02-00
Pages
749-51
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414144
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.19.2.749-751.1978 PMC Full Text View journal details
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