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PMID: 7009568 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores.

Journal of bacteriology ·Vol. 143 ·No. 2 ·1980-08-00 ·Pages 958-65

Harper JF, Clancy MJ, Magee PT

Abstract

Bulk ribonucleic acid (RNA) was isolated from mechanically disrupted ascospores of Saccharomyces cerevisiae. After two passes over an oligo (dT10) cellulose column, the portion which bound, called poly(A)(+), was characterized. It is heterodisperse in size with a mean molecular weight of approximately 4 X 10(5), but contains some species as large as 7 X 10(5). The base composition is similar to vegetative poly(A)(+) RNA. The polyadenylate segment is also heterogenous in size, ranging from 90 to 20 bases in length, with a peak at approximately 60 nucleotides in length. Pulse-labeling of asci with [3H-methyl]methionine yields two "caps," 7-methyl guanosine-5'-triphosphoryl-5'-adenosine (or guanosine) identical to that found in vegetative poly(A)(+) RNA. The poly(A)(+) RNA in spores is found in polyribosomes which are, on the average, smaller than vegetative ones. Long-term labeling studies indicate that the fraction of poly(A)(+) RNA in spores is similar to that in vegetative cells.

MeSH Terms
Base Sequence Molecular Weight Poly A/metabolism Polyribosomes/metabolism RNA Caps/analysis RNA, Fungal/metabolism RNA, Messenger/metabolism Saccharomyces cerevisiae/genetics,physiology Spores, Fungal
Chemicals
RNA Caps RNA, Fungal RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harper J F
Clancy M J
Magee P T
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.2.958-965.1980
Published
1980-08-00
Pages
958-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294400
Subset
IM
Grants
NIAID NIH HHS · AI 11730 · United States
External Links
PubMed source DOI: 10.1128/jb.143.2.958-965.1980 PMC Full Text View journal details
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