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PMID: 7008032 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA strand specificity in promoter recognition by RNA polymerase.

Nucleic acids research ·Vol. 8 ·No. 23 ·1980-12-11 ·Pages 5895-912

Park CS, Hillel Z, Wu CW

Abstract

DNA strand and enzyme subunit specificities involved in the interaction between E. coli RNA polymerase and T7 DNA were studied by photo-crosslinking techniques. In non-specific enzyme-DNA complexes, subunits, sigma, beta, and beta' were crosslinked to both strands of the DNA. Under conditions leading to specific enzyme-promoter complexes, however, only sigma and beta subunits were crosslinked. The sigma subunit was crosslinked preferentially to the non-sense strand at promoter sites. No such strand specificity was observed for the beta subunit. These results provide insight into the molecular mechanism of promoter recognition and indicate that the interaction between RNA polymerase and DNA template is different at promoters and at non-specific sites.

MeSH Terms
Binding Sites DNA, Viral/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Nucleic Acid Conformation Operon/radiation effects Protein Conformation/radiation effects Substrate Specificity T-Phages/metabolism Ultraviolet Rays
Chemicals
DNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Park C S
Hillel Z
Wu C W
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.23.5895
Published
1980-12-11
Pages
5895-912
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324349
Subset
IM
Grants
NIGMS NIH HHS · GM 28069 · United States
External Links
PubMed source DOI: 10.1093/nar/8.23.5895 PMC Full Text View journal details
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