Escherichia coli RNA-polymerase binding sites on DNA are only 14 base pairs long and are located between sequences that are very rich in AplusT.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3091-5

Giacomoni PU, Le Talaer JY, Le Pecq JB

Abstract

E. coli DNA-dependent RNA-polymerase binding sites on DNAs of T5, T7, and lambda coliphages have been isolated according to three different methods in order to analyze the binding sites themselves as well as the nearest neighboring regions. It is shown that the binding sites are regions that are rather rich in G+C, are about 14 base pairs long and are located between DNA sequences highly enriched in A+T. The biological implications of this result are discussed.

MeSH Terms

Adenine Nucleotides/isolation & purification Base Sequence Binding Sites Coliphages DNA Viruses/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism DNA-Directed RNA Polymerases/metabolism Electrophoresis, Polyacrylamide Gel Ethidium/metabolism Nucleic Acid Conformation Nucleic Acid Denaturation Nucleotides/isolation & purification,metabolism Phosphorus Radioisotopes Spectrometry, Fluorescence Thymine Nucleotides/isolation & purification Transcription, Genetic

Chemicals

Adenine Nucleotides DNA, Single-Stranded DNA, Viral Nucleotides Phosphorus Radioisotopes Thymine Nucleotides DNA-Directed RNA Polymerases Ethidium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Giacomoni P U

Le Talaer J Y

Le Pecq J B

References (15)

15 references, click to expand

A neighbor-interaction analysis of the hypochromism and spectra of DNA.
J Mol Biol. 1965 Sep;13(2):407-27 PMID: 5867028
Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.
J Biol Chem. 1967 Apr 10;242(7):1541-7 PMID: 4290246
Studies on the binding of RNA polymerase to polynucleotides.
J Mol Biol. 1966 Dec 28;22(2):199-209 PMID: 5972769
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.
J Mol Biol. 1967 Jul 14;27(1):87-106 PMID: 6033613
Pyrimidine clusters on the transcribing strand of DNA and their possible role in the initiation of RNA synthesis.
Cold Spring Harb Symp Quant Biol. 1966;31:123-7 PMID: 4966069
Self-complementary oligoribonucleotides: effects of helix defects and guanylic acid-cytidylic acid base pairs.
J Mol Biol. 1971 Apr 28;57(2):217-29 PMID: 5579620
Purification and base composition analysis of phage lambda early promoters.
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3211-5 PMID: 4332016
Angular alteration of the DNA helix by E. coli RNA polymerase.
Nat New Biol. 1972 Oct 11;239(93):167-70 PMID: 4573694
Effect of DNA conformation on ribosomal RNA synthesis in vitro.
Nat New Biol. 1973 Jun 6;243(127):161-3 PMID: 4576627
Isolation of Escherichia coli RNA polymerase binding sites on T5 and T7 DNA: further evidence for sigma-dependent recognition of A-T-rich DNA sequences.
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2911-5 PMID: 4355373
The nucleotide sequence of the lac operator.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4 PMID: 4587255
Identification of the nucleotide sequences involved in the interaction between Escherichia coli 5 RNA and specific 50 S subunit proteins.
J Mol Biol. 1973 Jun 15;77(1):133-52 PMID: 4588880
Different binding of RNA polymerase to individual promoters.
Mol Gen Genet. 1973 Nov 2;126(2):165-75 PMID: 4360101
On the initiation of transcription by DNA-dependent RNA polymerase from Escherichia coli.
Eur J Biochem. 1973 Dec 17;40(2):367-73 PMID: 4592650
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J Mol Biol. 1962 Jul;5:109-18 PMID: 14470099

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.8.3091

Published

1974-08-00

Pages

3091-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388627

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.8.3091 PMC Full Text View journal details

Analysis Services

Escherichia coli RNA-polymerase binding sites on DNA are only 14 base pairs long and are located between sequences that are very rich in AplusT.

Contact

WeChat Official Account