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PMID: 7007826 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The dnaA gene product is not required during stable chromosome replication in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 180 ·No. 3 ·1980-00-00 ·Pages 617-20

Cieśla Z, Jonczyk P

Abstract

Exposure of Escherichia coli dnaA strains to UV light results in a transient resumption of chromosome replication at 42 degrees C, the temperature restrictive to these mutants. Capability of dnaA mutants to replicate DNA at 42 degrees C can be stabilized, however, when either protein or RNA synthesis is inhibited 60 min after UV irradiation. DNA synthesis proceeds for several hours under these conditions. These results indicate that dnaA dependent transcription is not involved in initiation of chromosome cycles during stable DNA synthesis.

MeSH Terms
Chromosomes, Bacterial/radiation effects DNA Replication/radiation effects DNA, Bacterial/biosynthesis Escherichia coli/genetics,ultrastructure Hot Temperature Light Mutation Time Factors Transcription, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cieśla Z
Jonczyk P
References (10)
10 references, click to expand
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    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:937-46 PMID: 385236
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    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:537-49 PMID: 383381
  9. Rifampicin and chloramphenicol effects on DNA replication in ultraviolet-damaged Escherichia coli B/r WP2 thy trp.
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    J Mol Biol. 1961 Apr;3:144-55 PMID: 13764647
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268069
Published
1980-00-00
Pages
617-20
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
PHS HHS · 05-089 N · United States
External Links
PubMed source DOI: 10.1007/BF00268069
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