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PMID: 7002903 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Prophage induction in a permeabilized cell system: induction by deoxyribonucleases and the role of recBC-deoxyribonuclease.

Journal of bacteriology ·Vol. 144 ·No. 3 ·1980-12-00 ·Pages 1061-7

Irbe RM, Oishi M

Abstract

Permeabilized cells able to induce prophage were obtained by plasmolysis and preincubation of the cells in a reaction mixture which allows protein synthesis. These cells became permeable to low-molecular-weight proteins and oligonucleotides. We found that deoxyribonucleases (pancreatic deoxyribonuclease and micrococcal nuclease) triggered prophage (phi 80) induction. This deoxyribonuclease-triggered induction was completely dependent upon the presence of functional recBC genes in the lysogen, regardless of the recombination proficiency determined by recBC and sbcB genes. The possible role of recBC-deoxyribonuclease in prophage induction and recombination is discussed.

MeSH Terms
Alleles Coliphages/growth & development Deoxyribonucleases/pharmacology Escherichia coli/genetics Lysogeny Mutation Recombination, Genetic Virus Activation
Chemicals
Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Irbe R M
Oishi M
References (11)
11 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.144.3.1061-1067.1980
Published
1980-12-00
Pages
1061-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294771
Subset
IM
Grants
NIGMS NIH HHS · GM-21073 · United States
External Links
PubMed source DOI: 10.1128/jb.144.3.1061-1067.1980 PMC Full Text View journal details
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