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PMID: 6991478 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Tumbling chemotaxis mutants of Escherichia coli: possible gene-dependent effect of methionine starvation.

Journal of bacteriology ·Vol. 142 ·No. 2 ·1980-05-00 ·Pages 527-34

Kondoh H

Abstract

Some mutants defective in chemotaxis show incessant tumbling behavior and are called tumbling mutants. Previously described tumbling mutations lie in two genes, cheB and cheZ (41.5 min on Escherichia coli map). Genetic analysis of various tumbling mutants, however, revealed that two more genetic loci, cheC (43 min) and cheE (99.2 min), could also mutate to produce tumbling mutants. The genetic map around cheC was revised: his flaP flaQ flaR flbD flaA (= cheC) flaE. flbD is a new gene. When cells were starved for methionine, the tumbling mutants changed their swimming behavior depending on the che gene mutated. cheZ mutants, like wild-type bacteria, ceased tumbling shortly after removal of methionine. The tumbling of cheB or cheE mutants was depressed after prolonged methionine starvation in the presence of a constant level of an attractant. cheC tumbling mutants appeared unique in that they did not cease tumbling even when cells were deprived of methionine. By contrast, arsenate treatment of the tumbling mutants resulted in smooth swimming of the cells in every case. These results suggest that two different processes are involved in regulation of tumbling; one requiring methionine and the other requiring some phosphorylated compound.

MeSH Terms
Arsenates/pharmacology Chemotaxis Chromosome Mapping Chromosomes, Bacterial Escherichia coli/genetics,physiology Genes Methionine/metabolism Mutation
Chemicals
Arsenates Methionine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kondoh H
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47 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.142.2.527-534.1980
Published
1980-05-00
Pages
527-34
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294018
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.142.2.527-534.1980 PMC Full Text View journal details
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