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PMID: 6984193 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Serum and epidermal growth factor transiently depolarize quiescent BSC-1 epithelial cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 24 ·1982-12-00 ·Pages 7783-7

Rothenberg P, Reuss L, Glaser L

Abstract

Continuous intracellular recording of membrane potential with microelectrodes in BSC-1 epithelial cells has been used to study the early ionic effects of the interaction of mitogens with cell surface receptors. Initial results show that (i) there is no significant difference in membrane potential between growing and quiescent cells, (ii) addition of epidermal growth factor or serum to quiescent BSC-1 cells induces a brief and transient depolarization, (iii) the mitogenic response of BSC-1 cells to epidermal growth factor and the transient depolarization show similar concentration dependences, and (iv) serum addition to quiescent BSC-1 cells induces a sustained increase in Na+ influx that is electroneutral and amiloride sensitive. Intracellular pH changes may be a primary event triggering the response of quiescent cells to mitogenic polypeptides.

MeSH Terms
Amiloride/pharmacology Animals Cell Membrane Permeability/drug effects Cells, Cultured Culture Media Epidermal Growth Factor/pharmacology Membrane Potentials/drug effects Sodium/physiology
Chemicals
Culture Media Epidermal Growth Factor Amiloride Sodium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rothenberg P
Reuss L
Glaser L
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.24.7783
Published
1982-12-00
Pages
7783-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347432
Subset
IM
Grants
NIADDK NIH HHS · AM 19580 · United States
NIGMS NIH HHS · GM 02016 · United States
NIGMS NIH HHS · GM 18405 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.24.7783 PMC Full Text View journal details
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