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PMID: 6972042 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Antigen-presenting cell function in induction of helper T cells for cytotoxic T-lymphocyte responses: evidence for antigen processing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1796-9

Weinberger O, Herrmann S, Mescher MF, Benacerraf B, Burakoff SJ

Abstract

We demonstrate that splenic adherent cells (SACs) play an active role in the presentation of H-2Kk antigen for an alloreactive cytotoxic T-lymphocyte (CTL) response. If antigen is incubated with SACs for 12 hr, they will provide maximal stimulation and present the antigen in the context of their Ia molecules. UV irradiation of these SACs, prior to the 12-hr incubation with H-2Kk antigen, abrogates this stimulatory capacity. Macrophage-bound antigen is not sufficient for stimulation of a response; a second signal is required as well, that, in our system, is provided by phorbol myristic acetate. The SACs are involved in the activation of helper T cells; however, they are not required for presentation of antigen to the precytotoxic T-lymphocyte, which requires two signals for activation, one provided by antigen and the other by a T-cell-derived helper factor.

MeSH Terms
Animals Antigens/analysis Cytotoxicity, Immunologic/radiation effects Immune Sera Liposomes Mice Mice, Inbred Strains T-Lymphocytes/immunology,radiation effects Ultraviolet Rays
Chemicals
Antigens Immune Sera Liposomes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Weinberger O
Herrmann S
Mescher M F
Benacerraf B
Burakoff S J
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1796
Published
1981-03-00
Pages
1796-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319221
Subset
IM
Grants
NCI NIH HHS · CA14723 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1796 PMC Full Text View journal details
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