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PMID: 4542806 Published · ppublish English Journal Article

Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes.

The Journal of experimental medicine ·Vol. 138 ·No. 5 ·1973-11-01 ·Pages 1194-212

Rosenthal AS, Shevach EM

Abstract

Antigen activation of DNA synthesis in immune thymus-derived lymphocytes of guinea pigs requires the cooperation of macrophages and lymphocytes. We have investigated the role of histocompatibility determinants in this macrophage-lymphocyte interaction using cells from inbred strain 2 and 13 guinea pigs. The data demonstrate that efficient presentation of macrophage-associated antigen to the lymphocyte requires identity between macrophage and lymphocyte at some portion of the major histocompatibility complex. The failure of allogeneic macrophages to effectively initiate immune lymphocyte proliferation was not the result of the presence of an inhibitor of blastogenesis released in mixtures of allogeneic cells, peculiarities of the antigen or lymphoid cells employed, nor differing kinetics of activation by allogeneic macrophages. In addition, data were presented that demonstrated that alloantisera inhibit lymphocyte DNA synthesis by functional interference with macrophage-lymphocyte interaction.

MeSH Terms
Animals Ascitic Fluid/cytology Binding Sites, Antibody Cell Division DNA/biosynthesis Guinea Pigs Histocompatibility Histocompatibility Testing Immune Sera Immunologic Memory Kinetics Lymphocyte Activation Lymphocytes/metabolism Macrophages/immunology Mitogens/pharmacology T-Lymphocytes/immunology
Chemicals
Immune Sera Mitogens DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rosenthal A S
Shevach E M
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32 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.138.5.1194
Published
1973-11-01
Pages
1194-212
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139433
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.138.5.1194 PMC Full Text View journal details
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