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PMID: 5762048 Published · ppublish English Journal Article

Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters.

The Journal of experimental medicine ·Vol. 129 ·No. 2 ·1969-02-01 ·Pages 351-62

Mosier DE

Abstract

Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.

MeSH Terms
Animals Antibody Formation Antigen-Antibody Reactions Carbon Isotopes Cell Aggregation Cell Membrane Culture Techniques Erythrocytes Lymphocytes/immunology Mice Mitosis Sheep Spleen/immunology Surface Properties Thymidine Time Factors
Chemicals
Carbon Isotopes Thymidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Mosier D E
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25 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.129.2.351
Published
1969-02-01
Pages
351-62
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138600
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.129.2.351 PMC Full Text View journal details
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