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PMID: 6956911 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assignment of the structural gene for the third component of human complement to chromosome 19.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 16 ·1982-08-00 ·Pages 5021-5

Whitehead AS, Solomon E, Chambers S, Bodmer WF, Povey S, Fey G

Abstract

The third component of complement (C3) is synthesized and secreted by cultured human primary fibroblasts. A monoclonal antibody having specificity for an antigenic determinant carried by human but not mouse C3 was used to study the continued expression of human C3 in three panels of independently derived human-mouse somatic cell hybrids. Expression of the human product was shown to segregate with human chromosome 19 and with no other chromosome or group of chromosomes. A unique-sequence human genomic C3 DNA clone was isolated and used as a probe in DNA hybridization experiments with DNA prepared from appropriate human-mouse somatic cell hybrids to confirm assignment of the human C3 gene to chromosome 19.

MeSH Terms
Chromosome Mapping Chromosomes Complement C3/genetics Gene Expression Regulation Genes Humans Hybrid Cells Nucleic Acid Hybridization
Chemicals
Complement C3
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Whitehead A S
Solomon E
Chambers S
Bodmer W F
Povey S
Fey G
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.16.5021
Published
1982-08-00
Pages
5021-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346818
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.16.5021 PMC Full Text View journal details
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